5m3h

X-ray diffraction
2.5Å resolution

Bat influenza A/H17N10 polymerase bound to four heptad repeats of serine 5 phosphorylated Pol II CTD

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (6 distinct):
Polymerase acidic protein Chain: A
Molecule details ›
Chain: A
Length: 728 amino acids
Theoretical weight: 84.24 KDa
Source organism: Influenza A virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: H6QM92 (Residues: 1-713; Coverage: 100%)
Gene name: PA
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PA
RNA-directed RNA polymerase catalytic subunit Chain: B
Molecule details ›
Chain: B
Length: 776 amino acids
Theoretical weight: 87.94 KDa
Source organism: Influenza A virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: H6QM91 (Residues: 1-756; Coverage: 100%)
Gene name: PB1
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PB1
Polymerase basic protein 2 Chain: C
Molecule details ›
Chain: C
Length: 797 amino acids
Theoretical weight: 90.17 KDa
Source organism: Influenza A virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: H6QM90 (Residues: 1-760; Coverage: 100%)
Gene name: PB2
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PB2
DNA-directed RNA polymerase II subunit RPB1 Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 28 amino acids
Theoretical weight: 3.22 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P24928 (Residues: 1713-1740; Coverage: 1%)
Gene names: POLR2, POLR2A
Sequence domains: RNA polymerase Rpb1 C-terminal repeat
RNA (5'-R(*UP*AP*UP*AP*CP*CP*UP*CP*UP*GP*CP*UP*U)-3') Chain: R
Molecule details ›
Chain: R
Length: 18 nucleotides
Theoretical weight: 5.58 KDa
RNA 5'-pAGUAGUAACAAGAGGG Chain: V
Molecule details ›
Chain: V
Length: 16 nucleotides
Theoretical weight: 5.25 KDa

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 269.49Å b: 147.52Å c: 88.48Å
α: 90° β: 97.22° γ: 90°
R-values:
R R work R free
0.212 0.21 0.245
Expression systems:
  • Trichoplusia ni
  • Not provided