PDBe 5lzt

Electron Microscopy
3.65Å resolution

Structure of the mammalian ribosomal termination complex with eRF1 and eRF3.

Released:
Related structures: EMD-4131

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 87-mer (preferred)
Entry contents:
80 distinct polypeptide molecules
7 distinct RNA molecules
Macromolecules (87 distinct):
60S ribosomal protein L6 Chain: E
Molecule details ›
Chain: E
Length: 291 amino acids
Theoretical weight: 33.03 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SKF7 (Residues: 1-291; Coverage: 100%)
Gene name: RPL6
Sequence domains:
eL8 Chain: G
Molecule details ›
Chain: G
Length: 319 amino acids
Theoretical weight: 36.22 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1STW0 (Residues: 1-242; Coverage: 100%)
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
40S ribosomal protein S3a Chain: BB
Molecule details ›
Chain: BB
Length: 264 amino acids
Theoretical weight: 30 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SS70 (Residues: 1-264; Coverage: 100%)
Gene names: LOC100342838, LOC100348981, RPS3A
Sequence domains: Ribosomal S3Ae family
eS4 Chain: EE
Molecule details ›
Chain: EE
Length: 263 amino acids
Theoretical weight: 29.65 KDa
Source organism: Oryctolagus cuniculus
40S ribosomal protein S6 Chain: GG
Molecule details ›
Chain: GG
Length: 249 amino acids
Theoretical weight: 28.75 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TM55 (Residues: 1-249; Coverage: 100%)
Gene names: LOC100339133, RPS6
Sequence domains: Ribosomal protein S6e
40S ribosomal protein S7 Chain: HH
Molecule details ›
Chain: HH
Length: 194 amino acids
Theoretical weight: 22.17 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SVB0 (Residues: 1-194; Coverage: 100%)
Gene name: RPS7
Sequence domains: Ribosomal protein S7e
40S ribosomal protein S8 Chain: II
Molecule details ›
Chain: II
Length: 208 amino acids
Theoretical weight: 24.26 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TJW1 (Residues: 1-208; Coverage: 100%)
Gene name: LOC100352057
Sequence domains: Ribosomal protein S8e
Ribosomal protein L8 Chain: A
Molecule details ›
Chain: A
Length: 257 amino acids
Theoretical weight: 28.09 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TT27 (Residues: 1-257; Coverage: 100%)
Gene name: RPL8
Sequence domains:
uL3 Chain: B
Molecule details ›
Chain: B
Length: 403 amino acids
Theoretical weight: 46.11 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TL06 (Residues: 1-403; Coverage: 100%)
Gene name: RPL3
Sequence domains: Ribosomal protein L3
Ribosomal protein L4 Chain: C
Molecule details ›
Chain: C
Length: 425 amino acids
Theoretical weight: 47.73 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SVW5 (Residues: 15-392; Coverage: 96%)
Gene name: RPL4
Sequence domains:
Ribosomal protein L11 Chain: J
Molecule details ›
Chain: J
Length: 178 amino acids
Theoretical weight: 20.29 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TUB8 (Residues: 1-178; Coverage: 100%)
Gene name: RPL11
Sequence domains:
uL6 Chain: H
Molecule details ›
Chain: H
Length: 192 amino acids
Theoretical weight: 21.87 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TX33 (Residues: 1-192; Coverage: 100%)
Sequence domains: Ribosomal protein L6
uS2 Chain: AA
Molecule details ›
Chain: AA
Length: 295 amino acids
Theoretical weight: 32.96 KDa
Source organism: Oryctolagus cuniculus
Ribosomal protein S3 Chain: DD
Molecule details ›
Chain: DD
Length: 243 amino acids
Theoretical weight: 26.72 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TNM3 (Residues: 39-281; Coverage: 87%)
Gene name: RPS3
Sequence domains:
Ribosomal protein S9 (Predicted) Chain: JJ
Molecule details ›
Chain: JJ
Length: 194 amino acids
Theoretical weight: 22.64 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: B7NZS8 (Residues: 1-194; Coverage: 100%)
Gene name: RPS9
Sequence domains:
uS5 Chain: CC
Molecule details ›
Chain: CC
Length: 293 amino acids
Theoretical weight: 31.33 KDa
Source organism: Oryctolagus cuniculus
Ribosomal protein S5 Chain: FF
Molecule details ›
Chain: FF
Length: 204 amino acids
Theoretical weight: 22.91 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TFM5 (Residues: 1-204; Coverage: 100%)
Gene name: RPS5
Sequence domains: Ribosomal protein S7p/S5e
Ribosomal protein S15a Chain: WW
Molecule details ›
Chain: WW
Length: 130 amino acids
Theoretical weight: 14.87 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TG89 (Residues: 1-130; Coverage: 100%)
Gene name: RPS15A
Sequence domains: Ribosomal protein S8
Ribosomal protein S16 Chain: QQ
Molecule details ›
Chain: QQ
Length: 146 amino acids
Theoretical weight: 16.48 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SGX4 (Residues: 27-172; Coverage: 85%)
Gene name: RPS16
Sequence domains: Ribosomal protein S9/S16
eL13 Chain: L
Molecule details ›
Chain: L
Length: 211 amino acids
Theoretical weight: 24.33 KDa
Source organism: Oryctolagus cuniculus
Ribosomal protein L14 Chain: M
Molecule details ›
Chain: M
Length: 218 amino acids
Theoretical weight: 23.87 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SZ12 (Residues: 1-218; Coverage: 100%)
Gene name: RPL14
Sequence domains: Ribosomal protein L14
Ribosomal protein L15 Chain: N
Molecule details ›
Chain: N
Length: 204 amino acids
Theoretical weight: 24.21 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1T0C1 (Residues: 1-204; Coverage: 100%)
Gene names: LOC100358370, RPL15
Sequence domains: Ribosomal L15
eL18 Chain: Q
Molecule details ›
Chain: Q
Length: 188 amino acids
Theoretical weight: 21.7 KDa
Source organism: Oryctolagus cuniculus
eL19 Chain: R
Molecule details ›
Chain: R
Length: 196 amino acids
Theoretical weight: 23.54 KDa
Source organism: Oryctolagus cuniculus
eL20 Chain: S
Molecule details ›
Chain: S
Length: 176 amino acids
Theoretical weight: 20.83 KDa
Source organism: Oryctolagus cuniculus
eL21 Chain: T
Molecule details ›
Chain: T
Length: 160 amino acids
Theoretical weight: 18.61 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SHQ2 (Residues: 1-160; Coverage: 100%)
Gene name: RPL21
Sequence domains: Ribosomal protein L21e
eL22 Chain: U
Molecule details ›
Chain: U
Length: 128 amino acids
Theoretical weight: 14.81 KDa
Source organism: Oryctolagus cuniculus
eL24 Chain: W
Molecule details ›
Chain: W
Length: 157 amino acids
Theoretical weight: 17.83 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SE28 (Residues: 1-157; Coverage: 100%)
Gene name: RPL24
Sequence domains: Ribosomal protein L24e
60S ribosomal protein L27 Chain: Z
Molecule details ›
Chain: Z
Length: 136 amino acids
Theoretical weight: 15.84 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TXF6 (Residues: 1-136; Coverage: 100%)
Gene names: LOC100356974, RPL27
Sequence domains:
eL28 Chain: r
Molecule details ›
Chain: r
Length: 137 amino acids
Theoretical weight: 15.78 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1U7L1 (Residues: 1-137; Coverage: 100%)
Gene name: LOC100358162
Sequence domains: Ribosomal L28e protein family
eL29 Chain: b
Molecule details ›
Chain: b
Length: 245 amino acids
Theoretical weight: 26.71 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SGR6 (Residues: 1-226; Coverage: 100%)
Gene name: RPL29
Sequence domains: Ribosomal L29e protein family
eL30 Chain: c
Molecule details ›
Chain: c
Length: 115 amino acids
Theoretical weight: 12.81 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TDL2 (Residues: 1-115; Coverage: 100%)
Gene name: RPL30
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
eL31 Chain: d
Molecule details ›
Chain: d
Length: 125 amino acids
Theoretical weight: 14.49 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SHG0 (Residues: 1-125; Coverage: 100%)
Gene name: RPL31
Sequence domains: Ribosomal protein L31e
eL32 Chain: e
Molecule details ›
Chain: e
Length: 135 amino acids
Theoretical weight: 15.9 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1U437 (Residues: 1-135; Coverage: 100%)
Gene names: LOC100345217, LOC100352536, LOC100353583, LOC100359042
Sequence domains: Ribosomal protein L32
eL33 Chain: f
Molecule details ›
Chain: f
Length: 110 amino acids
Theoretical weight: 12.58 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SF08 (Residues: 1-110; Coverage: 100%)
Gene name: RPL35A
Sequence domains: Ribosomal protein L35Ae
eL34 Chain: g
Molecule details ›
Chain: g
Length: 117 amino acids
Theoretical weight: 13.33 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1U945 (Residues: 11-126; Coverage: 92%)
Gene name: LOC100354714
Sequence domains: Ribosomal protein L34e
60S ribosomal protein L36 Chain: i
Molecule details ›
Chain: i
Length: 105 amino acids
Theoretical weight: 12.26 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TTQ5 (Residues: 1-105; Coverage: 100%)
Gene name: LOC100348796
Sequence domains: Ribosomal protein L36e
Ribosomal protein L37 Chain: j
Molecule details ›
Chain: j
Length: 97 amino acids
Theoretical weight: 11.11 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: U3KPD5 (Residues: 1-97; Coverage: 100%)
Gene name: RPL37
Sequence domains: Ribosomal protein L37e
eL38 Chain: k
Molecule details ›
Chain: k
Length: 70 amino acids
Theoretical weight: 8.24 KDa
Source organism: Oryctolagus cuniculus
eL39 Chain: l
Molecule details ›
Chain: l
Length: 51 amino acids
Theoretical weight: 6.46 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TTN1 (Residues: 1-51; Coverage: 100%)
Sequence domains: Ribosomal L39 protein
eL40 Chain: m
Molecule details ›
Chain: m
Length: 102 amino acids
Theoretical weight: 11.7 KDa
Source organism: Oryctolagus cuniculus
eL41 Chain: n
Molecule details ›
Chain: n
Length: 25 amino acids
Theoretical weight: 3.47 KDa
Source organism: Oryctolagus cuniculus
eL42 Chain: o
Molecule details ›
Chain: o
Length: 106 amino acids
Theoretical weight: 12.48 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1U344 (Residues: 1-106; Coverage: 100%)
Gene names: LOC100353469, LOC100358069, LOC100358359
Sequence domains: Ribosomal protein L44
eL43 Chain: p
Molecule details ›
Chain: p
Length: 92 amino acids
Theoretical weight: 10.3 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SY53 (Residues: 1-92; Coverage: 100%)
Gene name: LOC100351807
Sequence domains: Ribosomal L37ae protein family
Eukaryotic peptide chain release factor subunit 1 Chain: ii
Molecule details ›
Chain: ii
Length: 459 amino acids
Theoretical weight: 51.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62495 (Residues: 1-437; Coverage: 100%)
Gene names: ERF1, ETF1, RF1, SUP45L1
Sequence domains:
eS10 Chain: KK
Molecule details ›
Chain: KK
Length: 165 amino acids
Theoretical weight: 18.93 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TPV3 (Residues: 1-165; Coverage: 100%)
Gene name: LOC100348511
Sequence domains: Plectin/S10 domain
40S ribosomal protein S12 Chain: MM
Molecule details ›
Chain: MM
Length: 132 amino acids
Theoretical weight: 14.54 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SFR8 (Residues: 1-132; Coverage: 100%)
Gene name: RPS12
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
eS17 Chain: RR
Molecule details ›
Chain: RR
Length: 135 amino acids
Theoretical weight: 15.55 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TU13 (Residues: 1-135; Coverage: 100%)
Gene name: RPS17
Sequence domains: Ribosomal S17
eS19 Chain: TT
Molecule details ›
Chain: TT
Length: 145 amino acids
Theoretical weight: 16.11 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TN62 (Residues: 1-145; Coverage: 100%)
Gene name: LOC100345774
Sequence domains: Ribosomal protein S19e
eS21 Chain: VV
Molecule details ›
Chain: VV
Length: 83 amino acids
Theoretical weight: 9.12 KDa
Source organism: Oryctolagus cuniculus
eS24 Chain: YY
Molecule details ›
Chain: YY
Length: 130 amino acids
Theoretical weight: 15.11 KDa
Source organism: Oryctolagus cuniculus
eS25 Chain: ZZ
Molecule details ›
Chain: ZZ
Length: 125 amino acids
Theoretical weight: 13.78 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TDB3 (Residues: 1-125; Coverage: 100%)
Gene name: RPS25
Sequence domains: S25 ribosomal protein
eS26 Chain: aa
Molecule details ›
Chain: aa
Length: 115 amino acids
Theoretical weight: 13.05 KDa
Source organism: Oryctolagus cuniculus
40S ribosomal protein S27 Chain: bb
Molecule details ›
Chain: bb
Length: 84 amino acids
Theoretical weight: 9.48 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TZ76 (Residues: 1-84; Coverage: 100%)
Gene names: LOC100345044, LOC100345694, LOC100348261, RPS27
Sequence domains: Ribosomal protein S27
Ribosomal protein S28 Chain: cc
Molecule details ›
Chain: cc
Length: 69 amino acids
Theoretical weight: 7.86 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TIB4 (Residues: 1-69; Coverage: 100%)
Gene name: RPS28
Sequence domains: Ribosomal protein S28e
40S ribosomal protein S30 Chain: ee
Molecule details ›
Chain: ee
Length: 133 amino acids
Theoretical weight: 14.5 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1T8A2 (Residues: 1-133; Coverage: 100%)
Gene name: FAU
Sequence domains:
Ribosomal protein S27a Chain: ff
Molecule details ›
Chain: ff
Length: 156 amino acids
Theoretical weight: 18 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SK22 (Residues: 1-156; Coverage: 100%)
Gene name: RPS27A
Sequence domains:
uL10 Chain: s
Molecule details ›
Chain: s
Length: 318 amino acids
Theoretical weight: 34.38 KDa
Source organism: Oryctolagus cuniculus
Ribosomal protein L12 Chain: t
Molecule details ›
Chain: t
Length: 165 amino acids
Theoretical weight: 17.85 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SMR7 (Residues: 31-195; Coverage: 85%)
Gene name: RPL12
Sequence domains:
uL13 Chain: O
Molecule details ›
Chain: O
Length: 203 amino acids
Theoretical weight: 23.53 KDa
Source organism: Oryctolagus cuniculus
Ribosomal protein L23 Chain: V
Molecule details ›
Chain: V
Length: 140 amino acids
Theoretical weight: 14.89 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1T6D1 (Residues: 1-140; Coverage: 100%)
Gene name: RPL23
Sequence domains: Ribosomal protein L14p/L23e
uL15 Chain: a
Molecule details ›
Chain: a
Length: 148 amino acids
Theoretical weight: 16.62 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SNY0 (Residues: 1-148; Coverage: 100%)
Gene name: RPL27A
Sequence domains: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Ribosomal protein L10 (Predicted) Chain: I
Molecule details ›
Chain: I
Length: 214 amino acids
Theoretical weight: 24.64 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: B7NZQ2 (Residues: 1-214; Coverage: 100%)
Gene name: RPL10
Sequence domains: Ribosomal protein L16p/L10e
60S ribosomal protein L5 Chain: D
Molecule details ›
Chain: D
Length: 297 amino acids
Theoretical weight: 34.48 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SYJ6 (Residues: 2-297; Coverage: 100%)
Gene name: RPL5
Sequence domains:
uL22 Chain: P
Molecule details ›
Chain: P
Length: 184 amino acids
Theoretical weight: 21.44 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TVT6 (Residues: 4-187; Coverage: 98%)
Gene name: RPL17
Sequence domains: Ribosomal protein L22p/L17e
uL23 Chain: X
Molecule details ›
Chain: X
Length: 156 amino acids
Theoretical weight: 17.77 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SE76 (Residues: 1-156; Coverage: 100%)
Gene names: LOC100350855, LOC100358852, RPL23A
Sequence domains:
uL24 Chain: Y
Molecule details ›
Chain: Y
Length: 145 amino acids
Theoretical weight: 17.3 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SQH0 (Residues: 1-145; Coverage: 100%)
Gene name: RPL26
Sequence domains:
uL29 Chain: h
Molecule details ›
Chain: h
Length: 123 amino acids
Theoretical weight: 14.57 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SIT5 (Residues: 1-123; Coverage: 100%)
Gene name: RPL35
Sequence domains: Ribosomal L29 protein
uL30 Chain: F
Molecule details ›
Chain: F
Length: 247 amino acids
Theoretical weight: 29.2 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TUB1 (Residues: 1-247; Coverage: 100%)
Sequence domains:
uS10 Chain: UU
Molecule details ›
Chain: UU
Length: 119 amino acids
Theoretical weight: 13.4 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SIZ2 (Residues: 1-119; Coverage: 100%)
Gene name: LOC100354063
Sequence domains: Ribosomal protein S10p/S20e
Ribosomal protein S14 Chain: OO
Molecule details ›
Chain: OO
Length: 168 amino acids
Theoretical weight: 18.13 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1T1F0 (Residues: 1-156; Coverage: 100%)
Gene name: RPS14
Sequence domains: Ribosomal protein S11
uS12 Chain: XX
Molecule details ›
Chain: XX
Length: 143 amino acids
Theoretical weight: 15.84 KDa
Source organism: Oryctolagus cuniculus
uS13 Chain: SS
Molecule details ›
Chain: SS
Length: 152 amino acids
Theoretical weight: 17.76 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TPG3 (Residues: 1-152; Coverage: 100%)
Gene name: LOC100356144
Sequence domains: Ribosomal protein S13/S18
uS14 Chain: dd
Molecule details ›
Chain: dd
Length: 56 amino acids
Theoretical weight: 6.69 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1U7M4 (Residues: 1-56; Coverage: 100%)
Gene name: LOC100337712
Sequence domains: Ribosomal protein S14p/S29e
Ribosomal protein S13 Chain: NN
Molecule details ›
Chain: NN
Length: 151 amino acids
Theoretical weight: 17.26 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SP51 (Residues: 1-151; Coverage: 100%)
Gene name: RPS13
Sequence domains:
Ribosomal protein S11 Chain: LL
Molecule details ›
Chain: LL
Length: 158 amino acids
Theoretical weight: 18.47 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TRM4 (Residues: 1-158; Coverage: 100%)
Gene name: RPS11
Sequence domains:
uS19 Chain: PP
Molecule details ›
Chain: PP
Length: 145 amino acids
Theoretical weight: 17.05 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1U0Q2 (Residues: 1-145; Coverage: 100%)
Gene name: LOC103349305
Sequence domains: Ribosomal protein S19
RACK1 Chain: gg
Molecule details ›
Chain: gg
Length: 317 amino acids
Theoretical weight: 35.12 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SJB4 (Residues: 1-317; Coverage: 100%)
Gene name: GNB2L1
Sequence domains: WD domain, G-beta repeat
Eukaryotic peptide chain release factor GTP-binding subunit ERF3A Chain: jj
Molecule details ›
Chain: jj
Length: 637 amino acids
Theoretical weight: 68.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P15170 (Residues: 1-499; Coverage: 100%)
Gene names: ERF3A, GSPT1
Sequence domains:
Nascent chain Chain: 1
Molecule details ›
Chain: 1
Length: 15 amino acids
Theoretical weight: 1.79 KDa
Source organism: Oryctolagus cuniculus
E-site tRNA Chain: 3
Molecule details ›
Chain: 3
Length: 75 nucleotides
Theoretical weight: 24.1 KDa
Sequence domains: tRNA
P-site tRNA Chain: 2
Molecule details ›
Chain: 2
Length: 76 nucleotides
Theoretical weight: 24.44 KDa
Sequence domains: tRNA
5S ribosomal RNA Chain: 7
Molecule details ›
Chain: 7
Length: 120 nucleotides
Theoretical weight: 38.69 KDa
Sequence domains: 5S ribosomal RNA
18S Ribosomal RNA Chain: 9
Molecule details ›
Chain: 9
Length: 1869 nucleotides
Theoretical weight: 602.78 KDa
Sequence domains: Eukaryotic small subunit ribosomal RNA
28S ribosomal RNA Chain: 5
Molecule details ›
Chain: 5
Length: 3543 nucleotides
Theoretical weight: 1148.12 KDa
Sequence domains: Eukaryotic large subunit ribosomal RNA
5.8S ribosomal RNA Chain: 8
Molecule details ›
Chain: 8
Length: 156 nucleotides
Theoretical weight: 50.14 KDa
Sequence domains: 5.8S ribosomal RNA
mRNA (UGA stop codon) Chain: hh
Molecule details ›
Chain: hh
Length: 15 nucleotides
Theoretical weight: 4.75 KDa

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.65Å
Relevant EMDB volumes: EMD-4131
Expression system: Escherichia coli BL21(DE3)