5luf

Electron Microscopy
9.1Å resolution

Cryo-EM of bovine respirasome

Released:
Source organism: Bos taurus
Related structures: EMD-4107

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only 62-mer (preferred)
Entry contents:
51 distinct polypeptide molecules
Macromolecules (51 distinct):
Cytochrome b-c1 complex subunit 1, mitochondrial Chains: c, l
Molecule details ›
Chains: c, l
Length: 446 amino acids
Theoretical weight: 49.27 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P31800 (Residues: 35-480; Coverage: 93%)
Gene name: UQCRC1
Sequence domains:
Cytochrome b-c1 complex subunit 2, mitochondrial Chains: m, n
Molecule details ›
Chains: m, n
Length: 439 amino acids
Theoretical weight: 46.58 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P23004 (Residues: 15-453; Coverage: 97%)
Gene name: UQCRC2
Sequence domains:
Cytochrome b Chains: b, o
Molecule details ›
Chains: b, o
Length: 379 amino acids
Theoretical weight: 42.62 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00157 (Residues: 1-379; Coverage: 100%)
Gene names: COB, CYTB, MT-CYB, MTCYB
Sequence domains:
Cytochrome c1, heme protein, mitochondrial Chains: d, p
Molecule details ›
Chains: d, p
Length: 241 amino acids
Theoretical weight: 27.32 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00125 (Residues: 85-325; Coverage: 74%)
Gene name: CYC1
Sequence domains: Cytochrome C1 family
Cytochrome b-c1 complex subunit Rieske, mitochondrial Chains: e, q
Molecule details ›
Chains: e, q
Length: 196 amino acids
Theoretical weight: 21.64 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P13272 (Residues: 79-274; Coverage: 72%)
Gene name: UQCRFS1
Sequence domains:
Cytochrome b-c1 complex subunit 7 Chains: f, r
Molecule details ›
Chains: f, r
Length: 110 amino acids
Theoretical weight: 13.37 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00129 (Residues: 2-111; Coverage: 99%)
Gene name: UQCRB
Sequence domains: Ubiquinol-cytochrome C reductase complex 14kD subunit
Cytochrome b-c1 complex subunit 8 Chains: g, s
Molecule details ›
Chains: g, s
Length: 81 amino acids
Theoretical weight: 9.61 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P13271 (Residues: 2-82; Coverage: 99%)
Gene name: UQCRQ
Sequence domains: UcrQ family
Cytochrome b-c1 complex subunit 6, mitochondrial Chains: h, t
Molecule details ›
Chains: h, t
Length: 78 amino acids
Theoretical weight: 9.19 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00126 (Residues: 14-91; Coverage: 86%)
Gene name: UQCRH
Sequence domains: Ubiquinol-cytochrome C reductase hinge protein
Cytochrome b-c1 complex subunit 9 Chains: i, u
Molecule details ›
Chains: i, u
Length: 78 amino acids
Theoretical weight: 7.96 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P13272 (Residues: 1-78; Coverage: 29%)
Gene name: UQCRFS1
Sequence domains: Ubiquinol-cytochrome c reductase 8 kDa, N-terminal
Cytochrome b-c1 complex subunit 9 Chains: j, v
Molecule details ›
Chains: j, v
Length: 62 amino acids
Theoretical weight: 7.21 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00130 (Residues: 2-63; Coverage: 97%)
Gene name: UQCR10
Sequence domains: Ubiquinol-cytochrome C reductase, UQCRX/QCR9 like
Cytochrome b-c1 complex subunit 10 Chains: k, w
Molecule details ›
Chains: k, w
Length: 56 amino acids
Theoretical weight: 6.37 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P07552 (Residues: 1-56; Coverage: 100%)
Gene names: UQCR, UQCR11
Sequence domains: Ubiquinol-cytochrome C reductase complex, 6.4kD protein
Cytochrome c oxidase subunit 1 Chain: x
Molecule details ›
Chain: x
Length: 514 amino acids
Theoretical weight: 57.07 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00396 (Residues: 1-514; Coverage: 100%)
Gene names: COI, COXI, MT-CO1, MTCO1
Sequence domains: Cytochrome C and Quinol oxidase polypeptide I
Cytochrome c oxidase subunit 2 Chain: y
Molecule details ›
Chain: y
Length: 227 amino acids
Theoretical weight: 26.04 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P68530 (Residues: 1-227; Coverage: 100%)
Gene names: COII, COX2, COXII, MT-CO2, MTCO2
Sequence domains:
Cytochrome c oxidase subunit 3 Chain: z
Molecule details ›
Chain: z
Length: 261 amino acids
Theoretical weight: 29.94 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00415 (Residues: 1-261; Coverage: 100%)
Gene names: COIII, COXIII, MT-CO3, MTCO3
Sequence domains: Cytochrome c oxidase subunit III
Cytochrome c oxidase subunit 4 isoform 1, mitochondrial Chain: 1
Molecule details ›
Chain: 1
Length: 147 amino acids
Theoretical weight: 17.18 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00423 (Residues: 23-169; Coverage: 87%)
Gene names: COX4, COX4I1
Sequence domains: Cytochrome c oxidase subunit IV
Cytochrome c oxidase subunit 5A, mitochondrial Chain: 2
Molecule details ›
Chain: 2
Length: 109 amino acids
Theoretical weight: 12.45 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00426 (Residues: 44-152; Coverage: 72%)
Gene name: COX5A
Sequence domains: Cytochrome c oxidase subunit Va
Cytochrome c oxidase subunit 5B, mitochondrial Chain: 3
Molecule details ›
Chain: 3
Length: 98 amino acids
Theoretical weight: 10.68 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00428 (Residues: 32-129; Coverage: 76%)
Gene name: COX5B
Cytochrome c oxidase subunit 6A2, mitochondrial Chain: 4
Molecule details ›
Chain: 4
Length: 84 amino acids
Theoretical weight: 9.45 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P07471 (Residues: 13-96; Coverage: 87%)
Gene names: COX6A, COX6A2
Cytochrome c oxidase subunit 6B1 Chain: 5
Molecule details ›
Chain: 5
Length: 85 amino acids
Theoretical weight: 10.04 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00429 (Residues: 2-86; Coverage: 99%)
Gene names: COX6B, COX6B1
Sequence domains: Cytochrome oxidase c subunit VIb
Cytochrome c oxidase subunit 6C Chain: 6
Molecule details ›
Chain: 6
Length: 73 amino acids
Theoretical weight: 8.49 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P04038 (Residues: 2-74; Coverage: 99%)
Gene name: COX6C
Sequence domains: Cytochrome c oxidase subunit VIc
Cytochrome c oxidase subunit 7A1, mitochondrial Chain: 7
Molecule details ›
Chain: 7
Length: 59 amino acids
Theoretical weight: 6.68 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P07470 (Residues: 22-80; Coverage: 74%)
Gene names: COX7A, COX7A1, COX7AH
Sequence domains: Cytochrome c oxidase subunit VII
Cytochrome c oxidase subunit 7B, mitochondrial Chain: 8
Molecule details ›
Chain: 8
Length: 56 amino acids
Theoretical weight: 6.37 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P13183 (Residues: 25-80; Coverage: 70%)
Gene name: COX7B
Cytochrome c oxidase subunit 7C, mitochondrial Chain: 9
Molecule details ›
Chain: 9
Length: 47 amino acids
Theoretical weight: 5.45 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00430 (Residues: 17-63; Coverage: 75%)
Gene names: COX7C, COX7CP1
Cytochrome c oxidase subunit 8B, mitochondrial Chain: 0
Molecule details ›
Chain: 0
Length: 46 amino acids
Theoretical weight: 4.97 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P10175 (Residues: 25-70; Coverage: 66%)
Gene names: COX8B, COX8H
Sequence domains: Cytochrome oxidase c subunit VIII
complex I Chain: A
Molecule details ›
Chain: A
Length: 111 amino acids
Theoretical weight: 9.46 KDa
Source organism: Bos taurus
complex I Chain: B
Molecule details ›
Chain: B
Length: 143 amino acids
Theoretical weight: 12.26 KDa
Source organism: Bos taurus
complex I Chain: C
Molecule details ›
Chain: C
Length: 154 amino acids
Theoretical weight: 13.12 KDa
Source organism: Bos taurus
complex I Chain: D
Molecule details ›
Chain: D
Length: 384 amino acids
Theoretical weight: 32.7 KDa
Source organism: Bos taurus
complex I Chain: E
Molecule details ›
Chain: E
Length: 159 amino acids
Theoretical weight: 13.62 KDa
Source organism: Bos taurus
complex I Chain: F
Molecule details ›
Chain: F
Length: 411 amino acids
Theoretical weight: 35.07 KDa
Source organism: Bos taurus
complex I Chain: G
Molecule details ›
Chain: G
Length: 538 amino acids
Theoretical weight: 46.06 KDa
Source organism: Bos taurus
complex I Chain: H
Molecule details ›
Chain: H
Length: 313 amino acids
Theoretical weight: 26.66 KDa
Source organism: Bos taurus
complex I Chain: I
Molecule details ›
Chain: I
Length: 162 amino acids
Theoretical weight: 13.95 KDa
Source organism: Bos taurus
complex I Chain: J
Molecule details ›
Chain: J
Length: 171 amino acids
Theoretical weight: 14.57 KDa
Source organism: Bos taurus
complex I Chain: K
Molecule details ›
Chain: K
Length: 84 amino acids
Theoretical weight: 7.17 KDa
Source organism: Bos taurus
complex I Chain: L
Molecule details ›
Chain: L
Length: 601 amino acids
Theoretical weight: 51.17 KDa
Source organism: Bos taurus
complex I Chain: M
Molecule details ›
Chain: M
Length: 453 amino acids
Theoretical weight: 38.57 KDa
Source organism: Bos taurus
complex I Chain: N
Molecule details ›
Chain: N
Length: 345 amino acids
Theoretical weight: 29.38 KDa
Source organism: Bos taurus
complex I Chain: O
Molecule details ›
Chain: O
Length: 220 amino acids
Theoretical weight: 18.74 KDa
Source organism: Bos taurus
complex I Chain: P
Molecule details ›
Chain: P
Length: 303 amino acids
Theoretical weight: 25.8 KDa
Source organism: Bos taurus
complex I Chain: Q
Molecule details ›
Chain: Q
Length: 85 amino acids
Theoretical weight: 7.25 KDa
Source organism: Bos taurus
complex I Chain: R
Molecule details ›
Chain: R
Length: 47 amino acids
Theoretical weight: 4.02 KDa
Source organism: Bos taurus
complex I Chain: S
Molecule details ›
Chain: S
Length: 80 amino acids
Theoretical weight: 6.83 KDa
Source organism: Bos taurus
complex I Chain: T
Molecule details ›
Chain: T
Length: 75 amino acids
Theoretical weight: 6.4 KDa
Source organism: Bos taurus
complex I Chain: V
Molecule details ›
Chain: V
Length: 71 amino acids
Theoretical weight: 6.06 KDa
Source organism: Bos taurus
complex I Chain: W
Molecule details ›
Chain: W
Length: 72 amino acids
Theoretical weight: 6.15 KDa
Source organism: Bos taurus
complex I Chain: X
Molecule details ›
Chain: X
Length: 330 amino acids
Theoretical weight: 28.1 KDa
Source organism: Bos taurus
complex I Chain: Y
Molecule details ›
Chain: Y
Length: 106 amino acids
Theoretical weight: 9.04 KDa
Source organism: Bos taurus
complex I Chain: a
Molecule details ›
Chain: a
Length: 142 amino acids
Theoretical weight: 12.1 KDa
Source organism: Bos taurus
complex I Chain: U
Molecule details ›
Chain: U
Length: 828 amino acids
Theoretical weight: 70.48 KDa
Source organism: Bos taurus
complex I Chain: Z
Molecule details ›
Chain: Z
Length: 625 amino acids
Theoretical weight: 53.21 KDa
Source organism: Bos taurus

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 9.1Å
Relevant EMDB volumes: EMD-4107