5lqw

Electron Microscopy
5.8Å resolution

yeast activated spliceosome

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 31-mer (preferred)
Entry contents:
27 distinct polypeptide molecules
4 distinct RNA molecules
Macromolecules (31 distinct):
Pre-mRNA-splicing factor 8 Chain: A
Pre-mRNA-splicing factor SNU114 Chain: B
Molecule details ›
Chain: B
Length: 1008 amino acids
Theoretical weight: 114.17 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P36048 (Residues: 1-1008; Coverage: 100%)
Gene names: GIN10, SNU114, YKL173W, YKL637
Sequence domains:
Pre-mRNA-splicing helicase BRR2 Chain: C
Molecule details ›
Chain: C
Length: 2163 amino acids
Theoretical weight: 246.47 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32639 (Residues: 1-2163; Coverage: 100%)
Gene names: BRR2, RSS1, SNU246, SYGP-ORF66, YER172C
Sequence domains:
Pre-mRNA-splicing factor SLT11 Chain: D
Molecule details ›
Chain: D
Length: 364 amino acids
Theoretical weight: 40.99 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38241 (Residues: 1-364; Coverage: 100%)
Gene names: ECM2, SLT11, YBR0614, YBR065C
Pre-mRNA-splicing factor BUD31 Chain: E
Molecule details ›
Chain: E
Length: 157 amino acids
Theoretical weight: 18.48 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P25337 (Residues: 1-157; Coverage: 100%)
Gene names: BUD31, CWC14, YCR063W, YCR63W, YCR903
Sequence domains: G10 protein
Pre-mRNA-splicing factor CWC2 Chain: F
Molecule details ›
Chain: F
Length: 339 amino acids
Theoretical weight: 38.49 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12046 (Residues: 1-339; Coverage: 100%)
Gene names: CWC2, D1041, NTC40, SLC3, YDL209C
Sequence domains:
Pre-mRNA-splicing factor CWC22 Chain: H
Molecule details ›
Chain: H
Length: 577 amino acids
Theoretical weight: 67.39 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P53333 (Residues: 1-577; Coverage: 100%)
Gene names: CWC22, YGR278W
Sequence domains: MA3 domain
U2 snRNP component IST3 Chain: J
Molecule details ›
Chain: J
Length: 148 amino acids
Theoretical weight: 17.12 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P40565 (Residues: 1-148; Coverage: 100%)
Gene names: IST3, SNU17, YIB5W, YIR005W
Sequence domains: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Pre-mRNA-splicing factor PRP46 Chain: K
Molecule details ›
Chain: K
Length: 451 amino acids
Theoretical weight: 50.77 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12417 (Residues: 1-451; Coverage: 100%)
Gene names: CWC1, NTC50, P2594, PRP46, YPL151C
Sequence domains: WD domain, G-beta repeat
Pre-mRNA-splicing factor CWC26 Chain: L
Molecule details ›
Chain: L
Length: 266 amino acids
Theoretical weight: 30.53 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P46947 (Residues: 1-266; Coverage: 100%)
Gene names: BUD13, CWC26, G1642, SLC7, YGL174W
Sequence domains: Pre-mRNA-splicing factor of RES complex
Pre-mRNA-processing protein 45 Chain: M
Molecule details ›
Chain: M
Length: 379 amino acids
Theoretical weight: 42.55 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P28004 (Residues: 1-379; Coverage: 100%)
Gene names: FUN20, PRP45, YAL032C
Sequence domains: SKIP/SNW domain
Pre-mRNA leakage protein 1 Chain: N
Molecule details ›
Chain: N
Length: 204 amino acids
Theoretical weight: 23.69 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q07930 (Residues: 1-204; Coverage: 100%)
Gene names: L1591, PML1, YLR016C
Sequence domains: FHA domain
Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein PRP2 Chain: O
Molecule details ›
Chain: O
Length: 876 amino acids
Theoretical weight: 99.95 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P20095 (Residues: 1-876; Coverage: 100%)
Gene names: N2048, PRP2, RNA2, YNR011C
Sequence domains:
Pre-mRNA-splicing factor SYF1 Chain: P
Molecule details ›
Chain: P
Length: 859 amino acids
Theoretical weight: 100.34 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q04048 (Residues: 1-859; Coverage: 100%)
Gene names: NTC90, SYF1, YDR416W
U2 snRNP component HSH155 Chain: Q
Molecule details ›
Chain: Q
Length: 971 amino acids
Theoretical weight: 110.17 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P49955 (Residues: 1-971; Coverage: 100%)
Gene names: HSH155, YM8021.14, YMR288W
Pre-mRNA-splicing factor CLF1 Chain: R
Molecule details ›
Chain: R
Length: 687 amino acids
Theoretical weight: 82.56 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12309 (Residues: 1-687; Coverage: 100%)
Gene names: CLF1, L2952, NTC77, SYF3, YLR117C
Sequence domains: HAT (Half-A-TPR) repeat
Pre-mRNA-splicing factor CEF1 Chain: W
Molecule details ›
Chain: W
Length: 590 amino acids
Theoretical weight: 67.84 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q03654 (Residues: 1-590; Coverage: 100%)
Gene names: CEF1, NTC85, YM8261.07, YMR213W
Sequence domains:
Pre-mRNA-splicing factor RSE1 Chain: X
Molecule details ›
Chain: X
Length: 1361 amino acids
Theoretical weight: 153.96 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q04693 (Residues: 1-1361; Coverage: 100%)
Gene names: RSE1, SAP130, YM9827.03C, YML049C
Sequence domains:
Pre-mRNA-splicing factor RDS3 Chain: Y
Molecule details ›
Chain: Y
Length: 107 amino acids
Theoretical weight: 12.28 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q06835 (Residues: 1-107; Coverage: 100%)
Gene names: P9513.2, RDS3, YPR094W
Sequence domains: PHF5-like protein
RDS3 complex subunit 10 Chain: Z
Molecule details ›
Chain: Z
Length: 85 amino acids
Theoretical weight: 10.05 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0C074 (Residues: 1-85; Coverage: 100%)
Gene names: RCP10, YNL138W-A, YSF3
Sequence domains: Splicing factor 3B subunit 10 (SF3b10)
Small nuclear ribonucleoprotein-associated protein B Chain: b
Molecule details ›
Chain: b
Length: 196 amino acids
Theoretical weight: 22.43 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P40018 (Residues: 1-196; Coverage: 100%)
Gene names: SMB1, YER029C
Sequence domains: LSM domain
Small nuclear ribonucleoprotein Sm D3 Chain: d
Molecule details ›
Chain: d
Length: 101 amino acids
Theoretical weight: 11.24 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P43321 (Residues: 1-101; Coverage: 100%)
Gene names: L9634.6, SMD3, YLR147C
Sequence domains: LSM domain
Small nuclear ribonucleoprotein E Chain: e
Molecule details ›
Chain: e
Length: 94 amino acids
Theoretical weight: 10.39 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12330 (Residues: 1-94; Coverage: 100%)
Gene names: SME1, YOR159C
Sequence domains: LSM domain
Small nuclear ribonucleoprotein F Chain: f
Molecule details ›
Chain: f
Length: 86 amino acids
Theoretical weight: 9.67 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P54999 (Residues: 1-86; Coverage: 100%)
Gene names: P9705.4, SMX3, YPR182W
Sequence domains: LSM domain
Small nuclear ribonucleoprotein G Chain: g
Molecule details ›
Chain: g
Length: 77 amino acids
Theoretical weight: 8.49 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P40204 (Residues: 1-77; Coverage: 100%)
Gene names: SMX2, SNP2, YFL017Bw, YFL017W-A
Sequence domains: LSM domain
Small nuclear ribonucleoprotein Sm D1 Chain: h
Molecule details ›
Chain: h
Length: 146 amino acids
Theoretical weight: 16.3 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q02260 (Residues: 1-146; Coverage: 100%)
Gene names: SMD1, YGR074W
Sequence domains: LSM domain
Small nuclear ribonucleoprotein Sm D2 Chain: j
Molecule details ›
Chain: j
Length: 110 amino acids
Theoretical weight: 12.88 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q06217 (Residues: 1-110; Coverage: 100%)
Gene names: L9328.5, SMD2, YLR275W
Sequence domains: LSM domain
U2 snRNA Chain: 2
Molecule details ›
Chain: 2
Length: 1175 nucleotides
Theoretical weight: 376.27 KDa
Sequence domains: U2 spliceosomal RNA
U5 snRNA Chain: 5
Molecule details ›
Chain: 5
Length: 179 nucleotides
Theoretical weight: 57.44 KDa
Sequence domains: U5 spliceosomal RNA
U6 snRNA Chain: 6
Molecule details ›
Chain: 6
Length: 112 nucleotides
Theoretical weight: 35.88 KDa
Sequence domains: U6 spliceosomal RNA
actin pre-mRNA Chain: 9
Molecule details ›
Chain: 9
Length: 572 nucleotides
Theoretical weight: 182.57 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.8Å
Relevant EMDB volumes: EMD-4099
Expression system: Escherichia coli