PDBe 5lmf

X-ray diffraction
2.15Å resolution

Function and Biology Details

Reaction catalysed:
5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] + H(2)O = N(7)-methylguanosine 5'-diphosphate + 5'-phospho-[mRNA]
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
m7GpppN-mRNA hydrolase Chains: C, D
Molecule details ›
Chains: C, D
Length: 17 amino acids
Theoretical weight: 1.84 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P53550 (Residues: 484-500; Coverage: 2%)
Gene names: DCP2, N1917, PSU1, YNL118C
DNA topoisomerase 2-associated protein PAT1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 369 amino acids
Theoretical weight: 42.28 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P25644 (Residues: 435-796; Coverage: 46%)
Gene names: MRT1, PAT1, YCR077C, YCR77C

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: I222
Unit cell:
a: 98.36Å b: 122.92Å c: 126.97Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.212 0.246
Expression systems:
  • Not provided
  • Escherichia coli