5ljo

Electron Microscopy
4.9Å resolution

E. coli BAM complex (BamABCDE) by cryoEM

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Outer membrane protein assembly factor BamB Chain: B
Molecule details ›
Chain: B
Length: 371 amino acids
Theoretical weight: 39.69 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P77774 (Residues: 22-392; Coverage: 100%)
Gene names: JW2496, b2512, bamB, yfgL
Sequence domains:
Outer membrane protein assembly factor BamC Chain: C
Molecule details ›
Chain: C
Length: 165 amino acids
Theoretical weight: 17.86 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A903 (Residues: 25-189; Coverage: 52%)
Gene names: JW2462, b2477, bamC, dapX, nlpB
Outer membrane protein assembly factor BamD Chain: D
Molecule details ›
Chain: D
Length: 218 amino acids
Theoretical weight: 25.01 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AC02 (Residues: 26-243; Coverage: 97%)
Gene names: JW2577, b2595, bamD, yfiO
Sequence domains: Outer membrane lipoprotein
Outer membrane protein assembly factor BamE Chain: E
Molecule details ›
Chain: E
Length: 87 amino acids
Theoretical weight: 9.73 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A937 (Residues: 24-110; Coverage: 93%)
Gene names: JW2598, b2617, bamE, smpA
Sequence domains: SmpA / OmlA family
Outer membrane protein assembly factor BamA Chain: A
Molecule details ›
Chain: A
Length: 783 amino acids
Theoretical weight: 87.78 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A940 (Residues: 24-806; Coverage: 99%)
Gene names: JW0172, b0177, bamA, yaeT, yzzN, yzzY
Sequence domains: Surface antigen variable number repeat

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.9Å
Relevant EMDB volumes: EMD-4061
Expression system: Escherichia coli