› 5lbm

X-ray diffraction
2.7Å resolution

The asymmetric tetrameric structure of the formaldehyde sensing transcriptional repressor FrmR from Escherichia coli

Released:
DOI: 10.2210/pdb5lbm/pdb
PDB entry 5lbm coloured by chain, front view.
PDB entry 5lbm coloured by chain, side view.
PDB entry 5lbm coloured by chain, top view.
Source organism: Escherichia coli O157:H7
Primary publication:
The mechanism of a formaldehyde-sensing transcriptional regulator.
Denby KJ, Iwig J, Bisson C, Westwood J, Rolfe MD, Sedelnikova SE, Higgins K, Maroney MJ, Baker PJ, Chivers PT, Green J
Sci Rep 6 38879 (2016)
PMID: 27934966

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186835 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional repressor FrmR Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 91 amino acids
Theoretical weight: 10.43 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8X5J3 (Residues: 1-91; Coverage: 100%)
Gene names: ECs0412, Z0457, frmR
Sequence domains: Metal-sensitive transcriptional repressor
Structure domains: Metal-sensitive repressor, helix protomer

Ligands and Environments

1 bound ligand:
Ligand FOR 2 x FOR
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P31
Unit cell:
a: 82.069Å b: 82.069Å c: 55.251Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.209 0.266
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Allosteric control of metal-responsive transcriptional regulators in bacteria.
Baksh et al. (2020)
3 more

3 mentions without citation

Metallochaperones and metalloregulation in bacteria.
Capdevila et al. (2017)
2 more