5lbm

X-ray diffraction
2.7Å resolution

The asymmetric tetrameric structure of the formaldehyde sensing transcriptional repressor FrmR from Escherichia coli

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional repressor FrmR Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 91 amino acids
Theoretical weight: 10.43 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8X5J3 (Residues: 1-91; Coverage: 100%)
Gene names: ECs0412, Z0457, frmR
Sequence domains: Metal-sensitive transcriptional repressor
Structure domains: Metal-sensitive repressor, helix protomer

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P31
Unit cell:
a: 82.069Å b: 82.069Å c: 55.251Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.209 0.266
Expression system: Escherichia coli BL21