5l6m

X-ray diffraction
1.9Å resolution

Structure of Caulobacter crescentus VapBC1 (VapB1deltaC:VapC1 form)

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SpoVT-AbrB domain-containing protein Chains: A, D, E, H, I, L
Molecule details ›
Chains: A, D, E, H, I, L
Length: 78 amino acids
Theoretical weight: 8.66 KDa
Source organism: Caulobacter vibrioides CB15
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9AC34 (Residues: 2-72; Coverage: 90%)
Gene name: CC_0032
Sequence domains: Antidote-toxin recognition MazE, bacterial antitoxin
Ribonuclease VapC Chains: B, C, F, G, J, K
Molecule details ›
Chains: B, C, F, G, J, K
Length: 128 amino acids
Theoretical weight: 14.15 KDa
Source organism: Caulobacter vibrioides CB15
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9AC35 (Residues: 1-128; Coverage: 100%)
Gene names: CC_0031, vapC
Sequence domains: PIN domain
Structure domains: 5'-nuclease

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: C2
Unit cell:
a: 94.4Å b: 63.6Å c: 184.7Å
α: 90° β: 94.1° γ: 90°
R-values:
R R work R free
0.198 0.196 0.24
Expression system: Escherichia coli BL21(DE3)