5l3s

X-ray diffraction
1.9Å resolution

Structure of the GTPase heterodimer of crenarchaeal SRP54 and FtsY

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Signal recognition particle 54 kDa protein Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 298 amino acids
Theoretical weight: 33.11 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97ZE7 (Residues: 1-292; Coverage: 65%)
Gene names: SSO0971, srp54
Sequence domains: SRP54-type protein, helical bundle domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Signal recognition particle receptor FtsY Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 296 amino acids
Theoretical weight: 33.44 KDa
Source organism: Sulfolobus acidocaldarius DSM 639
Expression system: Escherichia coli
UniProt:
  • Canonical: P27414 (Residues: 79-368; Coverage: 79%)
Gene names: Saci_1462, dpa, ftsY, sso
Sequence domains: SRP54-type protein, GTPase domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 51.196Å b: 199.672Å c: 124.869Å
α: 90° β: 92.95° γ: 90°
R-values:
R R work R free
0.174 0.173 0.216
Expression system: Escherichia coli