5l0q

X-ray diffraction
2.76Å resolution

Crystal structure of the complex between ADAM10 D+C domain and a conformation specific mAb 8C7.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Disintegrin and metalloproteinase domain-containing protein 10 Chains: A, D
Molecule details ›
Chains: A, D
Length: 203 amino acids
Theoretical weight: 21.78 KDa
Source organism: Bos taurus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q10741 (Residues: 455-646; Coverage: 26%)
Gene names: ADAM10, MADM
Sequence domains: Disintegrin
mAb 8C7 light chain Chains: B, E
Molecule details ›
Chains: B, E
Length: 214 amino acids
Theoretical weight: 23.71 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
mAb 8C7 heavy chain Chains: C, F
Molecule details ›
Chains: C, F
Length: 222 amino acids
Theoretical weight: 23.93 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 53.326Å b: 141.679Å c: 268.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.203 0.251
Expression system: Homo sapiens