PDBe 5kz5

Electron Microscopy
14.3Å resolution

Architecture of the Human Mitochondrial Iron-Sulfur Cluster Assembly Machinery: the Complex Formed by the Iron Donor, the Sulfur Donor, and the Scaffold

Released:

Function and Biology Details

Reactions catalysed:
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 36-mer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cysteine desulfurase, mitochondrial Chains: 1, 2, 3, 4, M, N, O, P, Q, R, S, T
Molecule details ›
Chains: 1, 2, 3, 4, M, N, O, P, Q, R, S, T
Length: 391 amino acids
Theoretical weight: 43.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli #1/H766
UniProt:
  • Canonical: Q9Y697 (Residues: 67-457; Coverage: 86%)
Gene names: HUSSY-08, NFS1, NIFS
Frataxin intermediate form Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 169 amino acids
Theoretical weight: 18.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli #1/H766
UniProt:
  • Canonical: Q16595 (Residues: 42-210; Coverage: 81%)
Gene names: FRDA, FXN, X25
Sequence domains: Frataxin-like domain
Iron-sulfur cluster assembly enzyme ISCU, mitochondrial Chains: a, b, c, d, e, f, g, h, i, j, k, l
Molecule details ›
Chains: a, b, c, d, e, f, g, h, i, j, k, l
Length: 118 amino acids
Theoretical weight: 12.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli #1/H766
UniProt:
  • Canonical: Q9H1K1 (Residues: 50-167; Coverage: 71%)
Gene names: ISCU, NIFUN

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 14.3Å
Relevant EMDB volumes: EMD-8301
Expression system: Escherichia coli #1/H766