› 5kte

X-ray diffraction
3.94Å resolution

Crystal structure of Deinococcus radiodurans MntH, an Nramp-family transition metal transporter

Released:
DOI: 10.2210/pdb5kte/pdb
PDB 5kte coloured by chain and viewed from the front.
PDB 5kte coloured by chain and viewed from the side.
PDB 5kte coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Crystal Structure and Conformational Change Mechanism of a Bacterial Nramp-Family Divalent Metal Transporter.
Bozzi AT, Bane LB, Weihofen WA, Singharoy A, Guillen ER, Ploegh HL, Schulten K, Gaudet R
Structure (2016)
PMID: 27839948

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Divalent metal cation transporter MntH Chain: A
Molecule details ›
Chain: A
Length: 420 amino acids
Theoretical weight: 45.26 KDa
Source organism: Deinococcus radiodurans R1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RTP8 (Residues: 26-436; Coverage: 94%)
Gene names: DR_1709, mntH
Sequence domains: Natural resistance-associated macrophage protein
Fab Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 213 amino acids
Theoretical weight: 22.57 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Fab Light Chain Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 23.51 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand OS 3 x OS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: I222
Unit cell:
a: 113.131Å b: 132.077Å c: 220.995Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.272 0.268 0.313
Expression systems:
  • Escherichia coli
  • Mus musculus

Quick links

Citations

11 citation in other articles

Unique structural features in an Nramp metal transporter impart substrate-specific proton cotransport and a kinetic bias to favor import.
Bozzi et al. (2019)
10 more

1 mention without citation

Designing yeast as plant-like hyperaccumulators for heavy metals.
Sun et al. (2019)