5kl4

X-ray diffraction
1.78Å resolution

Wilms Tumor Protein (WT1) ZnF2-4 Q369H in complex with formylated DNA

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Wilms tumor protein Chains: A, D
Molecule details ›
Chains: A, D
Length: 93 amino acids
Theoretical weight: 11.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19544 (Residues: 350-440; Coverage: 20%)
  • Best match: P19544-4 (Residues: 350-437)
Gene name: WT1
Sequence domains: Zinc finger, C2H2 type
Structure domains: Classic Zinc Finger
DNA (5'-D(*AP*GP*CP*GP*TP*GP*GP*GP*(5FC)P*GP*T)-3') Chains: B, E
Molecule details ›
Chains: B, E
Length: 11 nucleotides
Theoretical weight: 3.46 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*TP*AP*(5CM)P*GP*CP*CP*CP*AP*CP*GP*C)-3') Chains: C, F
Molecule details ›
Chains: C, F
Length: 11 nucleotides
Theoretical weight: 3.29 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments


2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P1
Unit cell:
a: 35.766Å b: 43.853Å c: 54.808Å
α: 81.95° β: 88.57° γ: 88.51°
R-values:
R R work R free
0.218 0.216 0.246
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided