5kl2

X-ray diffraction
1.69Å resolution

Wilms Tumor Protein (WT1) ZnF2-4 in complex with DNA

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Wilms tumor protein Chain: A
Molecule details ›
Chain: A
Length: 93 amino acids
Theoretical weight: 11.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19544 (Residues: 350-440; Coverage: 20%)
  • Best match: P19544-4 (Residues: 350-437)
Gene name: WT1
Sequence domains: Zinc finger, C2H2 type
Structure domains: Classic Zinc Finger
DNA (5'-D(*AP*GP*CP*GP*TP*GP*GP*GP*AP*GP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.45 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*TP*AP*CP*TP*CP*CP*CP*AP*CP*GP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 11 nucleotides
Theoretical weight: 3.25 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 70.107Å b: 65.089Å c: 35.702Å
α: 90° β: 93.42° γ: 90°
R-values:
R R work R free
0.166 0.164 0.193
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided