X-ray diffraction
1.1Å resolution

horse liver S48T alcohol dehydrogenase complexed with NAD and pentafluorobenzyl alcohol


Function and Biology Details

Reaction catalysed:
A primary alcohol + NAD(+) = an aldehyde + NADH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Alcohol dehydrogenase E chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 374 amino acids
Theoretical weight: 39.87 KDa
Source organism: Equus caballus
Expression system: Escherichia coli
  • Canonical: P00327 (Residues: 2-375; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

Cofactor: Ligand NAJ 2 x NAJ
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P1
Unit cell:
a: 44.44Å b: 51.59Å c: 92.56Å
α: 91.87° β: 103.06° γ: 110.33°
R R work R free
0.118 0.118 0.138
Expression system: Escherichia coli