X-ray diffraction
2.81Å resolution

Crystal Structure of a Variant Human Activation-induced Deoxycytidine Deaminase as an MBP fusion protein


Function and Biology Details

Reaction catalysed:
Cytosine in single-stranded DNA + H(2)O = uracil in single-stranded DNA + NH(3)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein; Single-stranded DNA cytosine deaminase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 546 amino acids
Theoretical weight: 61.46 KDa
Source organisms: Expression system: Escherichia coli
  • Canonical: Q9GZX7 (Residues: 23-183; Coverage: 81%)
  • Canonical: P0AEY0 (Residues: 27-393; Coverage: 99%)
Gene names: AICDA, AID, ECs5017, Z5632, malE
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 106.793Å b: 96.806Å c: 108.692Å
α: 90° β: 102.86° γ: 90°
R R work R free
0.185 0.183 0.232
Expression system: Escherichia coli