5jaj

X-ray diffraction
1.5Å resolution

Structure of chicken LGP2 witha 5'p 10-mer dsRNA and ADP-AlF4-Mg.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
RNA helicase Chain: A
Molecule details ›
Chain: A
Length: 681 amino acids
Theoretical weight: 77.52 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0YYQ5 (Residues: 2-674; Coverage: 100%)
Sequence domains:
Structure domains:
RNA (5'-R(P*GP*GP*UP*AP*CP*GP*UP*AP*CP*CP*C)-3') Chain: X
Molecule details ›
Chain: X
Length: 11 nucleotides
Theoretical weight: 3.48 KDa
Source organism: synthetic construct
Expression system: Not provided
RNA (5'-R(P*GP*GP*UP*AP*CP*GP*UP*AP*CP*C)-3') Chain: Y
Molecule details ›
Chain: Y
Length: 10 nucleotides
Theoretical weight: 3.18 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 69.59Å b: 97.05Å c: 122.52Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.135 0.133 0.168
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided