PDBe 5j7w

X-ray diffraction
2.5Å resolution

Enterococcus faecalis thymidylate synthase complex with methotrexate


Function and Biology Details

Reaction catalysed:
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Thymidylate synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 315 amino acids
Theoretical weight: 36.39 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q834R3 (Residues: 1-315; Coverage: 100%)
Gene names: EF_1576, thyA
Sequence domains: Thymidylate synthase
Structure domains: Thymidylate synthase/dCMP hydroxymethylase domain

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 72.227Å b: 94.144Å c: 96.293Å
α: 90° β: 94.14° γ: 90°
R R work R free
0.204 0.2 0.273
Expression system: Escherichia coli BL21(DE3)