5j2c

X-ray diffraction
2.1Å resolution

Ternary complex crystal structure of DNA polymerase Beta with C:A mismatch at the primer terminus

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
DNA polymerase beta Chain: A
Molecule details ›
Chain: A
Length: 335 amino acids
Theoretical weight: 38.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06746 (Residues: 1-335; Coverage: 100%)
Gene name: POLB
Sequence domains:
Structure domains:
Template Strand Chain: T
Molecule details ›
Chain: T
Length: 16 nucleotides
Theoretical weight: 4.81 KDa
Source organism: synthetic construct
Expression system: Not provided
Primer Strand Chain: P
Molecule details ›
Chain: P
Length: 10 nucleotides
Theoretical weight: 3.09 KDa
Source organism: synthetic construct
Expression system: Not provided
Downstream Primer Strand Chain: D
Molecule details ›
Chain: D
Length: 5 nucleotides
Theoretical weight: 1.54 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 50.8Å b: 80.28Å c: 55.49Å
α: 90° β: 107.7° γ: 90°
R-values:
R R work R free
0.212 0.212 0.266
Expression systems:
  • Escherichia coli
  • Not provided