PDBe 5iuj

X-ray diffraction
3.2Å resolution

Crystal structure of the DesK-DesR complex in the phosphotransfer state with low Mg2+ (20 mM)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Sensor histidine kinase DesK Chains: A, B, D, E
Molecule details ›
Chains: A, B, D, E
Length: 218 amino acids
Theoretical weight: 24.96 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O34757 (Residues: 154-370; Coverage: 59%)
Gene names: BSU19190, desK, yocF
Sequence domains: Histidine kinase
Transcriptional regulatory protein DesR Chains: C, F
Molecule details ›
Chains: C, F
Length: 139 amino acids
Theoretical weight: 15.11 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O34723 (Residues: 1-135; Coverage: 68%)
Gene names: BSU19200, desR, yocG
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P21
Unit cell:
a: 87.82Å b: 114.619Å c: 91.597Å
α: 90° β: 116.44° γ: 90°
R-values:
R R work R free
0.189 0.187 0.24
Expression system: Escherichia coli BL21(DE3)