› 5iuc

X-ray diffraction
1.25Å resolution

Crystal structure of the GspB siglec domain with sialyl T antigen bound

Released:
DOI: 10.2210/pdb5iuc/pdb
PDB entry 5iuc coloured by chain, front view.
PDB entry 5iuc coloured by chain, side view.
PDB entry 5iuc coloured by chain, top view.
Source organism: Streptococcus gordonii
Primary publication:
A structural model for binding of the serine-rich repeat adhesin GspB to host carbohydrate receptors.
Pyburn TM, Bensing BA, Xiong YQ, Melancon BJ, Tomasiak TM, Ward NJ, Yankovskaya V, Oliver KM, Cecchini G, Sulikowski GA, Tyska MJ, Sullam PM, Iverson TM
PLoS Pathog 7 e1002112 (2011)
PMID: 21765814

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188025 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Platelet binding protein GspB Chains: A, B
Molecule details ›
Chains: A, B
Length: 127 amino acids
Theoretical weight: 13.95 KDa
Source organism: Streptococcus gordonii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q939N5 (Residues: 399-521; Coverage: 4%)
Gene name: gspB
Structure domains: Immunoglobulin-like

Ligands and Environments

Carbohydrate polymer : NEW Components: A2G, GAL, SIA
Carbohydrate polymer
1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21212
Unit cell:
a: 67.67Å b: 66.547Å c: 55.854Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.156 0.178
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Biological roles of glycans.
Varki A. (2017)
10 more

4 mentions without citation

Mycoplasma genitalium adhesin P110 binds sialic-acid human receptors.
Aparicio et al. (2018)
3 more