5itz

X-ray diffraction
2.2Å resolution

Crystal structure of the SAC domain of CPAP in a complex with Tubulin and Darpin

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-160564 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha-1B chain Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P81947 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Structure domains:
Tubulin beta-2B chain Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 50 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Sequence domains:
Structure domains:
Centromere protein J Chain: D
Molecule details ›
Chain: D
Length: 129 amino acids
Theoretical weight: 14.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HC77 (Residues: 311-422; Coverage: 8%)
Gene names: CENPJ, CPAP, LAP, LIP1
Designed ankyrin repeat protein (DARPIN) D1 Chain: F
Molecule details ›
Chain: F
Length: 127 amino acids
Theoretical weight: 13.36 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 61.08Å b: 85.34Å c: 98.69Å
α: 90° β: 91.77° γ: 90°
R-values:
R R work R free
0.179 0.177 0.217
Expression system: Escherichia coli BL21(DE3)