5igq

X-ray diffraction
3.9Å resolution

WD40 domain of Human E3 Ubiquitin Ligase COP1 (RFWD2) bound to peptide from Trib1

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
E3 ubiquitin-protein ligase COP1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 353 amino acids
Theoretical weight: 39.93 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q8NHY2 (Residues: 386-731; Coverage: 47%)
Gene names: COP1, RFWD2, RNF200
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Tribbles homolog 1 Chain: U
Molecule details ›
Chain: U
Length: 11 amino acids
Theoretical weight: 1.32 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q96RU8 (Residues: 354-364; Coverage: 3%)
Gene names: C8FW, GIG2, TRB1, TRIB1
Tribbles homolog 1 Chains: V, W, X, Y, Z
Molecule details ›
Chains: V, W, X, Y, Z
Length: 8 amino acids
Theoretical weight: 950 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q96RU8 (Residues: 354-361; Coverage: 2%)
Gene names: C8FW, GIG2, TRB1, TRIB1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C222
Unit cell:
a: 247.646Å b: 249.576Å c: 124.512Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.228 0.277
Expression systems:
  • Trichoplusia ni
  • Not provided