5igo

X-ray diffraction
1.6Å resolution

WD40 domain of Arabidopsis thaliana E3 Ubiquitin Ligase COP1 in complex with peptide from Trib1

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase COP1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 336 amino acids
Theoretical weight: 38.17 KDa
Source organism: Arabidopsis thaliana
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P43254 (Residues: 349-675; Coverage: 48%)
Gene names: At2g32950, COP1, T21L14.11
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Tribbles homolog 1 Chains: U, V, W, X
Molecule details ›
Chains: U, V, W, X
Length: 8 amino acids
Theoretical weight: 950 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q96RU8 (Residues: 354-361; Coverage: 2%)
Gene names: C8FW, GIG2, TRB1, TRIB1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 78.516Å b: 87.469Å c: 94.61Å
α: 90° β: 92.55° γ: 90°
R-values:
R R work R free
0.191 0.189 0.213
Expression systems:
  • Trichoplusia ni
  • Not provided