5i2d

X-ray diffraction
4.4Å resolution

Crystal structure of T. thermophilus TTHB099 class II transcription activation complex: TAP-RPo

Released:
Source organism: Thermus thermophilus HB8
Primary publication:
Structural basis of transcription activation.
Science 352 1330-3 (2016)
PMID: 27284196

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 11-mer (preferred)
Entry contents:
6 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (9 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B, L, M
Molecule details ›
Chains: A, B, L, M
Length: 315 amino acids
Theoretical weight: 35.06 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHR6 (Residues: 1-315; Coverage: 100%)
Gene names: TTHA1664, rpoA
Sequence domains:
DNA-directed RNA polymerase subunit beta Chains: C, N
Molecule details ›
Chains: C, N
Length: 1119 amino acids
Theoretical weight: 125.44 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q8RQE9 (Residues: 1-1119; Coverage: 100%)
Gene names: TTHA1813, rpoB
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chains: D, O
Molecule details ›
Chains: D, O
Length: 1524 amino acids
Theoretical weight: 171 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q8RQE8 (Residues: 1-1524; Coverage: 100%)
Gene names: TTHA1812, rpoC
Sequence domains:
DNA-directed RNA polymerase subunit omega Chains: E, P
Molecule details ›
Chains: E, P
Length: 99 amino acids
Theoretical weight: 11.53 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q8RQE7 (Residues: 1-99; Coverage: 100%)
Gene names: TTHA1561, rpoZ
Sequence domains: RNA polymerase Rpb6
RNA polymerase sigma factor SigA Chains: F, Q
Molecule details ›
Chains: F, Q
Length: 443 amino acids
Theoretical weight: 50.77 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SKW1 (Residues: 1-423; Coverage: 100%)
Gene names: TTHA0532, sigA
Sequence domains:
Transcriptional regulator LdrP Chains: G, H, R, S
Molecule details ›
Chains: G, H, R, S
Length: 215 amino acids
Theoretical weight: 24.34 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53W63 (Residues: 1-195; Coverage: 100%)
Gene name: TTHB099
Sequence domains:
DNA (72-MER) Chains: I, T
Molecule details ›
Chains: I, T
Length: 72 nucleotides
Theoretical weight: 22.23 KDa
DNA (72-MER) Chains: J, U
Molecule details ›
Chains: J, U
Length: 72 nucleotides
Theoretical weight: 22.34 KDa
RNA (5'-R(*UP*CP*GP*A)-3') Chains: K, V
Molecule details ›
Chains: K, V
Length: 4 nucleotides
Theoretical weight: 1.24 KDa

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 171.487Å b: 105.446Å c: 374.585Å
α: 90° β: 102.39° γ: 90°
R-values:
R R work R free
0.242 0.241 0.284
Expression system: Escherichia coli