X-ray diffraction
2.1Å resolution

X-Ray Structure of Unbound Porcine Epidemic Diarrhea Virus 3CLpro


Function and Biology Details

Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
3C-like proteinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 301 amino acids
Theoretical weight: 32.69 KDa
Source organism: Porcine epidemic diarrhea virus
Expression system: Escherichia coli
  • Canonical: U6BPB2 (Residues: 2998-3298; Coverage: 7%)
Gene name: ORF1a
Structure domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 58.586Å b: 76.292Å c: 119.169Å
α: 90° β: 90° γ: 90°
R R work R free
0.208 0.204 0.269
Expression system: Escherichia coli