› 5htd

X-ray diffraction
2.5Å resolution

Recombinant bovine beta-lactoglobulin variant L1A/I2S with endogenous ligand (sBlgB#1)

Released:
DOI: 10.2210/pdb5htd/pdb
PDB 5htd coloured by chain and viewed from the front.
PDB 5htd coloured by chain and viewed from the side.
PDB 5htd coloured by chain and viewed from the top.
Source organism: Bos taurus
Primary publication:
Engineered β-Lactoglobulin Produced in E. coli: Purification, Biophysical and Structural Characterisation.
Loch JI, Bonarek P, Tworzydło M, Polit A, Hawro B, Łach A, Ludwin E, Lewiński K
OpenAccess logo Mol Biotechnol 58 605-618 (2016)
PMID: 27380951

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactoglobulin Chain: A
Molecule details ›
Chain: A
Length: 162 amino acids
Theoretical weight: 18.23 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02754 (Residues: 17-178; Coverage: 100%)
Gene name: LGB
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

1 bound ligand:
Ligand MYR 1 x MYR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: Agilent SuperNova
Spacegroup: P3221
Unit cell:
a: 53.486Å b: 53.486Å c: 111.039Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.201 0.272
Expression system: Escherichia coli BL21(DE3)

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Citations

1 citations in other articles

Methyl-selective isotope labeling using α-ketoisovalerate for the yeast Pichia pastoris recombinant protein expression system.
Suzuki et al. (2018)