› 5hpg

X-ray diffraction
1.66Å resolution

STRUCTURE AND LIGAND DETERMINANTS OF THE RECOMBINANT KRINGLE 5 DOMAIN OF HUMAN PLASMINOGEN

Released:
DOI: 10.2210/pdb5hpg/pdb
PDB entry 5hpg coloured by chain, front view.
PDB entry 5hpg coloured by chain, side view.
PDB entry 5hpg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and ligand binding determinants of the recombinant kringle 5 domain of human plasminogen.
Chang Y, Mochalkin I, McCance SG, Cheng B, Tulinsky A, Castellino FJ
Biochemistry 37 3258-71 (1998)
PMID: 9521645

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Lys-|-Xaa > Arg-|-Xaa, higher selectivity than trypsin. Converts fibrin into soluble products.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133236 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Plasmin heavy chain A, short form Chains: A, B
Molecule details ›
Chains: A, B
Length: 84 amino acids
Theoretical weight: 9.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00747 (Residues: 480-563; Coverage: 11%)
Gene name: PLG
Sequence domains: Kringle domain
Structure domains: Plasminogen Kringle 4

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P21212
Unit cell:
a: 77.43Å b: 79.2Å c: 30.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.166 not available
Expression system: Escherichia coli

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Citations

7 review citations

Disulfide bonds as switches for protein function.
Hogg PJ. (2003)
6 more

6 mentions without citation

Plasminogen alleles influence susceptibility to invasive aspergillosis.
Zaas et al. (2008)
5 more