X-ray diffraction
2.22Å resolution

Crystal structure of T5Fen in complex intact substrate and metal ions.


Function and Biology Details

Reaction catalysed:
Exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 5'-phosphates
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Flap endonuclease Chains: A, B
Molecule details ›
Chains: A, B
Length: 272 amino acids
Theoretical weight: 31.28 KDa
Source organism: Escherichia virus T5
Expression system: Escherichia coli
  • Canonical: P06229 (Residues: 20-291; Coverage: 94%)
Gene names: D15, T5.130, T5p128, exo5
Sequence domains:
Structure domains:
DNA (5'-D(*AP*AP*AP*AP*GP*CP*GP*TP*AP*CP*GP*C)-3') Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 12 nucleotides
Theoretical weight: 3.68 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P212121
Unit cell:
a: 44.72Å b: 109.94Å c: 127.34Å
α: 90° β: 90° γ: 90°
R R work R free
0.185 0.183 0.234
Expression systems:
  • Escherichia coli
  • Not provided