5hkq

X-ray diffraction
2Å resolution

Crystal structure of CDI complex from Escherichia coli STEC_O31

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
tRNA nuclease CdiA Chain: A
Molecule details ›
Chain: A
Length: 143 amino acids
Theoretical weight: 15.48 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1S4NYE3 (Residues: 3111-3253; Coverage: 4%)
Gene names: ECSTECO31_4009, cdiA
Immunity protein CdiI Chain: I
Molecule details ›
Chain: I
Length: 129 amino acids
Theoretical weight: 15.46 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1S4NYE4 (Residues: 1-121; Coverage: 100%)
Gene names: ECSTECO31_4008, cdiI
Sequence domains: CDI immunity protein

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P65
Unit cell:
a: 89.431Å b: 89.431Å c: 76.155Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.169 0.167 0.208
Expression system: Escherichia coli BL21(DE3)