5hc9

X-ray diffraction
2.9Å resolution

Thermotoga maritima CCA-adding enzyme complexed with tRNA_CCA

Released:

Function and Biology Details

Reaction catalysed:
(1a) a tRNA precursor + CTP = a tRNA with a 3' cytidine end + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
tRNA nucleotidyl transferase-related protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 441 amino acids
Theoretical weight: 51.53 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WZH4 (Residues: 437-863; Coverage: 50%)
Gene name: TM_0715
Sequence domains:
Structure domains:
tRNAphe Chains: C, D
Molecule details ›
Chains: C, D
Length: 76 nucleotides
Theoretical weight: 24.48 KDa
Source organism: Thermotoga maritima
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P21
Unit cell:
a: 98.18Å b: 96.19Å c: 108.58Å
α: 90° β: 110.3° γ: 90°
R-values:
R R work R free
0.217 0.215 0.264
Expression systems:
  • Escherichia coli
  • Not provided