› 5has

X-ray diffraction
2.65Å resolution

Crystal structure of the N-terminal DCB-HUS domain of T. terrestris Sec7

Released:
DOI: 10.2210/pdb5has/pdb
PDB entry 5has coloured by chain, front view.
PDB entry 5has coloured by chain, side view.
PDB entry 5has coloured by chain, top view.
Source organism: Thermothielavioides terrestris
Primary publication:
The Sec7 N-terminal regulatory domains facilitate membrane-proximal activation of the Arf1 GTPase.
Richardson BC, Halaby SL, Gustafson MA, Fromme JC
Elife 5 (2016)
PMID: 26765562

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-102998 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sec7 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 468 amino acids
Theoretical weight: 51.89 KDa
Source organism: Thermothielavioides terrestris
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A140UHP8 (Residues: 1-458; Coverage: 100%)
Gene name: Sec7
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 62.472Å b: 132.024Å c: 247.664Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.257
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Who's in control? Principles of Rab GTPase activation in endolysosomal membrane trafficking and beyond.
Borchers et al. (2021)
2 more

3 mentions without citation

Structural Insights into Arl1-Mediated Targeting of the Arf-GEF BIG1 to the trans-Golgi.
Galindo et al. (2016)
2 more