5gq1

X-ray diffraction
2.49Å resolution

Crystal structure of 2C helicase from enterovirus 71 (EV71)

Released:
Source organism: Enterovirus A71
Primary publication:
Crystal structure of 2C helicase from enterovirus 71.
Sci Adv 3 e1602573 (2017)
PMID: 28508043

Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
NTP + H(2)O = NDP + phosphate
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein 2C Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 214 amino acids
Theoretical weight: 23.54 KDa
Source organism: Enterovirus A71
Expression system: Escherichia coli
UniProt:
  • Canonical: B9VUU3 (Residues: 1227-1440; Coverage: 10%)
Sequence domains: RNA helicase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NFPSS BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 52.109Å b: 159.654Å c: 75.606Å
α: 90° β: 90.16° γ: 90°
R-values:
R R work R free
0.232 0.229 0.286
Expression system: Escherichia coli