PDBe 5gaf

Electron Microscopy
4.3Å resolution

RNC in complex with SRP

Released:
Source organism: Escherichia coli
Related structures: EMD-8002

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 36-mer (preferred)
Entry contents:
32 distinct polypeptide molecules
4 distinct RNA molecules
Macromolecules (36 distinct):
Alkaline phosphatase Chain: k
Molecule details ›
Chain: k
Length: 18 amino acids
Theoretical weight: 1.98 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P00634 (Residues: 1-18; Coverage: 0%)
Gene names: JW0374, b0383, phoA
50S ribosomal protein L2 Chain: C
Molecule details ›
Chain: C
Length: 271 amino acids
Theoretical weight: 29.66 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60422 (Residues: 2-272; Coverage: 99%)
Gene names: JW3279, b3317, rplB
Sequence domains:
50S ribosomal protein L3 Chain: D
Molecule details ›
Chain: D
Length: 209 amino acids
Theoretical weight: 22.28 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60438 (Residues: 1-209; Coverage: 100%)
Gene names: JW3282, b3320, rplC
Sequence domains: Ribosomal protein L3
50S ribosomal protein L4 Chain: E
Molecule details ›
Chain: E
Length: 201 amino acids
Theoretical weight: 22.12 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60723 (Residues: 1-201; Coverage: 100%)
Gene names: JW3281, b3319, eryA, rplD
Sequence domains: Ribosomal protein L4/L1 family
50S ribosomal protein L5 Chain: F
Molecule details ›
Chain: F
Length: 177 amino acids
Theoretical weight: 20.07 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P62399 (Residues: 2-178; Coverage: 99%)
Gene names: JW3270, b3308, rplE
Sequence domains:
50S ribosomal protein L6 Chain: G
Molecule details ›
Chain: G
Length: 176 amino acids
Theoretical weight: 18.8 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG55 (Residues: 2-177; Coverage: 99%)
Gene names: JW3267, b3305, rplF
Sequence domains: Ribosomal protein L6
50S ribosomal protein L9 Chain: H
Molecule details ›
Chain: H
Length: 149 amino acids
Theoretical weight: 15.79 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7R1 (Residues: 1-149; Coverage: 100%)
Gene names: JW4161, b4203, rplI
Sequence domains:
50S ribosomal protein L10 Chain: I
Molecule details ›
Chain: I
Length: 125 amino acids
Theoretical weight: 13.48 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7J3 (Residues: 2-126; Coverage: 76%)
Gene names: JW3948, b3985, rplJ
Sequence domains: Ribosomal protein L10
50S ribosomal protein L11 Chain: J
Molecule details ›
Chain: J
Length: 134 amino acids
Theoretical weight: 14.02 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7J7 (Residues: 8-141; Coverage: 94%)
Gene names: JW3946, b3983, relC, rplK
Sequence domains:
50S ribosomal protein L13 Chain: K
Molecule details ›
Chain: K
Length: 142 amino acids
Theoretical weight: 16.05 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AA10 (Residues: 1-142; Coverage: 100%)
Gene names: JW3200, b3231, rplM
Sequence domains: Ribosomal protein L13
50S ribosomal protein L14 Chain: L
Molecule details ›
Chain: L
Length: 123 amino acids
Theoretical weight: 13.57 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADY3 (Residues: 1-123; Coverage: 100%)
Gene names: JW3272, b3310, rplN
Sequence domains: Ribosomal protein L14p/L23e
50S ribosomal protein L15 Chain: M
Molecule details ›
Chain: M
Length: 144 amino acids
Theoretical weight: 15.01 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P02413 (Residues: 1-144; Coverage: 100%)
Gene names: JW3263, b3301, rplO
Sequence domains: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
50S ribosomal protein L16 Chain: N
Molecule details ›
Chain: N
Length: 136 amino acids
Theoretical weight: 15.31 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADY7 (Residues: 1-136; Coverage: 100%)
Gene names: JW3275, b3313, rplP
Sequence domains: Ribosomal protein L16p/L10e
50S ribosomal protein L17 Chain: O
Molecule details ›
Chain: O
Length: 125 amino acids
Theoretical weight: 14.19 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG44 (Residues: 1-125; Coverage: 98%)
Gene names: JW3256, b3294, rplQ
Sequence domains: Ribosomal protein L17
50S ribosomal protein L18 Chain: P
Molecule details ›
Chain: P
Length: 117 amino acids
Theoretical weight: 12.79 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0C018 (Residues: 1-117; Coverage: 100%)
Gene names: JW3266, b3304, rplR
Sequence domains: Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
50S ribosomal protein L19 Chain: Q
Molecule details ›
Chain: Q
Length: 114 amino acids
Theoretical weight: 13.03 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7K6 (Residues: 2-115; Coverage: 99%)
Gene names: JW2587, b2606, rplS
Sequence domains: Ribosomal protein L19
50S ribosomal protein L20 Chain: R
Molecule details ›
Chain: R
Length: 117 amino acids
Theoretical weight: 13.4 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7L3 (Residues: 2-118; Coverage: 99%)
Gene names: JW1706, b1716, pdzA, rplT
Sequence domains: Ribosomal protein L20
50S ribosomal protein L21 Chain: S
Molecule details ›
Chain: S
Length: 103 amino acids
Theoretical weight: 11.59 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG48 (Residues: 1-103; Coverage: 100%)
Gene names: JW3153, b3186, rplU
Sequence domains: Ribosomal prokaryotic L21 protein
50S ribosomal protein L22 Chain: T
Molecule details ›
Chain: T
Length: 110 amino acids
Theoretical weight: 12.25 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P61175 (Residues: 1-110; Coverage: 100%)
Gene names: JW3277, b3315, eryB, rplV
Sequence domains: Ribosomal protein L22p/L17e
50S ribosomal protein L23 Chain: U
Molecule details ›
Chain: U
Length: 95 amino acids
Theoretical weight: 10.78 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADZ0 (Residues: 2-96; Coverage: 95%)
Gene names: JW3280, b3318, rplW
Sequence domains: Ribosomal protein L23
50S ribosomal protein L24 Chain: V
Molecule details ›
Chain: V
Length: 102 amino acids
Theoretical weight: 11.08 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60624 (Residues: 2-103; Coverage: 98%)
Gene names: JW3271, b3309, rplX
Sequence domains:
50S ribosomal protein L25 Chain: W
Molecule details ›
Chain: W
Length: 94 amino acids
Theoretical weight: 10.71 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P68919 (Residues: 1-94; Coverage: 100%)
Gene names: JW2173, b2185, rplY
Sequence domains: Ribosomal L25p family
50S ribosomal protein L27 Chain: X
Molecule details ›
Chain: X
Length: 76 amino acids
Theoretical weight: 8.28 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7L8 (Residues: 10-85; Coverage: 89%)
Gene names: JW3152, b3185, rpmA
50S ribosomal protein L28 Chain: Y
Molecule details ›
Chain: Y
Length: 77 amino acids
Theoretical weight: 8.9 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7M2 (Residues: 2-78; Coverage: 99%)
Gene names: JW3612, b3637, rpmB
Sequence domains: Ribosomal L28 family
50S ribosomal protein L29 Chain: Z
Molecule details ›
Chain: Z
Length: 62 amino acids
Theoretical weight: 7.16 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7M6 (Residues: 2-63; Coverage: 98%)
Gene names: JW3274, b3312, rpmC
Sequence domains: Ribosomal L29 protein
50S ribosomal protein L30 Chain: a
Molecule details ›
Chain: a
Length: 58 amino acids
Theoretical weight: 6.42 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG51 (Residues: 2-59; Coverage: 98%)
Gene names: JW3264, b3302, rpmD
Sequence domains: Ribosomal protein L30p/L7e
50S ribosomal protein L32 Chain: b
Molecule details ›
Chain: b
Length: 56 amino acids
Theoretical weight: 6.33 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7N4 (Residues: 2-57; Coverage: 98%)
Gene names: JW1075, b1089, rpmF
Sequence domains: Ribosomal L32p protein family
50S ribosomal protein L33 Chain: c
Molecule details ›
Chain: c
Length: 51 amino acids
Theoretical weight: 5.93 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7N9 (Residues: 4-54; Coverage: 93%)
Gene names: JW3611, b3636, rpmG
Sequence domains: Ribosomal protein L33
50S ribosomal protein L34 Chain: d
Molecule details ›
Chain: d
Length: 46 amino acids
Theoretical weight: 5.4 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7P5 (Residues: 1-46; Coverage: 100%)
Gene names: JW3680, b3703, rimA, rpmH, ssaF
Sequence domains: Ribosomal protein L34
50S ribosomal protein L35 Chain: e
Molecule details ›
Chain: e
Length: 64 amino acids
Theoretical weight: 7.18 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7Q1 (Residues: 2-65; Coverage: 99%)
Gene names: JW1707, b1717, rpmI
Sequence domains: Ribosomal protein L35
50S ribosomal protein L36 Chain: f
Molecule details ›
Chain: f
Length: 38 amino acids
Theoretical weight: 4.38 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7Q6 (Residues: 1-38; Coverage: 100%)
Gene names: JW3261, b3299, rpmJ
Sequence domains: Ribosomal protein L36
Signal recognition particle protein Chain: i
Molecule details ›
Chain: i
Length: 398 amino acids
Theoretical weight: 43.55 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AGD7 (Residues: 1-434; Coverage: 84%)
Gene names: JW5414, b2610, ffh
Sequence domains:
tRNA CCAend Chain: 2
Molecule details ›
Chain: 2
Length: 3 nucleotides
Theoretical weight: 895 Da
SRP 4.5S RNA Chain: 1
Molecule details ›
Chain: 1
Length: 113 nucleotides
Theoretical weight: 36.5 KDa
Sequence domains: Bacterial small signal recognition particle RNA
5S ribosomal RNA Chain: B
Molecule details ›
Chain: B
Length: 120 nucleotides
Theoretical weight: 38.79 KDa
Sequence domains: 5S ribosomal RNA
23S ribosomal RNA Chain: A
Molecule details ›
Chain: A
Length: 2883 nucleotides
Theoretical weight: 934.97 KDa
Sequence domains: Bacterial large subunit ribosomal RNA

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.3Å
Relevant EMDB volumes: EMD-8002