PDBe 5g5d

X-ray diffraction
3Å resolution

Crystal Structure of the CohScaC2-XDocCipA type II complex from Clostridium thermocellum

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cell surface glycoprotein 2 Chain: A
Molecule details ›
Chain: A
Length: 171 amino acids
Theoretical weight: 18.95 KDa
Source organism: Hungateiclostridium thermocellum AD2
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q06853 (Residues: 205-364; Coverage: 24%)
Gene name: Cthe_3079
Sequence domains: Cohesin domain
Structure domains: Immunoglobulin-like
Cellulosomal-scaffolding protein A Chain: B
Molecule details ›
Chain: B
Length: 164 amino acids
Theoretical weight: 17.78 KDa
Source organism: Hungateiclostridium thermocellum ATCC 27405
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q06851 (Residues: 1691-1853; Coverage: 9%)
Gene names: Cthe_3077, cipA
Sequence domains: Dockerin type I domain
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: I222
Unit cell:
a: 52.36Å b: 125.805Å c: 130.467Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.261 0.256 0.303
Expression system: Escherichia coli BL21