5fur

Electron Microscopy
8.5Å resolution

Structure of human TFIID-IIA bound to core promoter DNA

Released:
Source organism: Homo sapiens
Related structures: EMD-3305

Function and Biology Details

Reactions catalysed:
ATP + a protein = ADP + a phosphoprotein
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Entry contents:
9 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (11 distinct):
TATA-box-binding protein Chain: A
Molecule details ›
Chain: A
Length: 339 amino acids
Theoretical weight: 37.73 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P20226 (Residues: 1-339; Coverage: 100%)
Gene names: GTF2D1, TBP, TF2D, TFIID
Sequence domains: Transcription factor TFIID (or TATA-binding protein, TBP)
Transcription initiation factor IIA alpha chain Chain: B
Molecule details ›
Chain: B
Length: 43 amino acids
Theoretical weight: 5.1 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P52655 (Residues: 9-51; Coverage: 11%)
Gene names: GTF2A1, TF2A1
Transcription initiation factor IIA beta chain Chain: C
Molecule details ›
Chain: C
Length: 47 amino acids
Theoretical weight: 5.59 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P52655 (Residues: 330-376; Coverage: 13%)
Gene names: GTF2A1, TF2A1
Transcription initiation factor IIA subunit 2 Chain: D
Molecule details ›
Chain: D
Length: 97 amino acids
Theoretical weight: 11.28 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P52657 (Residues: 3-99; Coverage: 89%)
Gene names: GTF2A2, TF2A2
Transcription initiation factor TFIID subunit 1 Chain: G
Molecule details ›
Chain: G
Length: 1893 amino acids
Theoretical weight: 215.15 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P21675 (Residues: 1-177, 178-1872; Coverage: 100%)
  • Best match: P21675-12 (Residues: 1-177, 199-1895)
Gene names: BA2R, CCG1, CCGS, TAF1, TAF2A
Sequence domains:
Transcription initiation factor TFIID subunit 7 Chain: H
Molecule details ›
Chain: H
Length: 349 amino acids
Theoretical weight: 40.33 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q15545 (Residues: 1-349; Coverage: 100%)
Gene names: TAF2F, TAF7, TAFII55
Sequence domains: TAFII55 protein conserved region
Transcription initiation factor TFIID subunit 2 Chain: I
Molecule details ›
Chain: I
Length: 1199 amino acids
Theoretical weight: 137.16 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q6P1X5 (Residues: 1-1199; Coverage: 100%)
Gene names: CIF150, TAF2, TAF2B
Transcription initiation factor TFIID subunit 6 Chains: J, K
Molecule details ›
Chains: J, K
Length: 677 amino acids
Theoretical weight: 72.75 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P49848 (Residues: 1-677; Coverage: 100%)
Gene names: TAF2E, TAF6, TAFII70
Sequence domains:
Transcription initiation factor TFIID subunit 8 Chain: L
Molecule details ›
Chain: L
Length: 310 amino acids
Theoretical weight: 34.3 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q7Z7C8 (Residues: 1-310; Coverage: 100%)
Gene names: TAF8, TAFII43, TBN
Sequence domains:
SUPER CORE PROMOTER Chain: E
Molecule details ›
Chain: E
Length: 89 nucleotides
Theoretical weight: 27.66 KDa
SUPER CORE PROMOTER Chain: F
Molecule details ›
Chain: F
Length: 93 nucleotides
Theoretical weight: 28.49 KDa

Ligands and Environments

No bound ligands
2 modified residues:

Experiments and Validation Details

Entry percentile scores
Resolution: 8.5Å
Relevant EMDB volumes: EMD-3305