5flg

X-ray diffraction
2.04Å resolution

Crystal structure of the 6-carboxyhexanoate-CoA ligase (BioW)from Bacillus subtilis in complex with AMPPNP

Released:

Function and Biology Details

Reaction catalysed:
ATP + 6-carboxyhexanoate + CoA = AMP + diphosphate + 6-carboxyhexanoyl-CoA
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
6-carboxyhexanoate--CoA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 260 amino acids
Theoretical weight: 29.67 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P53559 (Residues: 4-259; Coverage: 99%)
Gene names: BSU30240, bioW
Sequence domains: 6-carboxyhexanoate--CoA ligase

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 49.59Å b: 77.9Å c: 166Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.202 0.232
Expression system: Escherichia coli