5fkw

Electron Microscopy
7.3Å resolution

cryo-EM structure of the E. coli replicative DNA polymerase complex bound to DNA (DNA polymerase III alpha, beta, epsilon)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
DNA polymerase III subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 1160 amino acids
Theoretical weight: 130.09 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P10443 (Residues: 1-1160; Coverage: 100%)
Gene names: JW0179, b0184, dnaE, polC
Sequence domains:
Beta sliding clamp Chains: B, C
Molecule details ›
Chains: B, C
Length: 366 amino acids
Theoretical weight: 40.63 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A988 (Residues: 1-366; Coverage: 100%)
Gene names: JW3678, b3701, dnaN
Sequence domains:
DNA polymerase III subunit epsilon Chain: D
Molecule details ›
Chain: D
Length: 243 amino acids
Theoretical weight: 27.12 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03007 (Residues: 1-243; Coverage: 100%)
Gene names: JW0205, b0215, dnaQ, mutD
Sequence domains: Exonuclease
PRIMER-TEMPLATE DUPLEX DNA Chain: P
Molecule details ›
Chain: P
Length: 25 nucleotides
Theoretical weight: 7.8 KDa
PRIMER-TEMPLATE DUPLEX DNA Chain: T
Molecule details ›
Chain: T
Length: 29 nucleotides
Theoretical weight: 8.8 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 7.3Å
Relevant EMDB volumes: EMD-3202
Expression system: Escherichia coli BL21(DE3)