5fjx

X-ray diffraction
2.45Å resolution

Yeast delta-COP-I mu-homology domain complexed with Gcs1 WxxF peptide

Released:
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural basis for the binding of tryptophan-based motifs by δ-COP.
Proc. Natl. Acad. Sci. U.S.A. 112 14242-7 (2015)
PMID: 26578768

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Coatomer subunit delta Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 270 amino acids
Theoretical weight: 30.02 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P43621 (Residues: 282-546; Coverage: 49%)
Gene names: RET2, YFR051C
ADP-ribosylation factor GTPase-activating protein GCS1 Chains: D, E
Molecule details ›
Chains: D, E
Length: 8 amino acids
Theoretical weight: 1.07 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P35197 (Residues: 345-352; Coverage: 2%)
Gene names: GCS1, YDL226C

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 70.765Å b: 84.855Å c: 148.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.197 0.287
Expression system: Escherichia coli