› 5fjx

X-ray diffraction
2.45Å resolution

Yeast delta-COP-I mu-homology domain complexed with Gcs1 WxxF peptide

Released:
DOI: 10.2210/pdb5fjx/pdb
PDB 5fjx coloured by chain and viewed from the front.
PDB 5fjx coloured by chain and viewed from the side.
PDB 5fjx coloured by chain and viewed from the top.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural basis for the binding of tryptophan-based motifs by δ-COP.
Suckling RJ, Poon PP, Travis SM, Majoul IV, Hughson FM, Evans PR, Duden R, Owen DJ
Proc Natl Acad Sci U S A 112 14242-7 (2015)
PMID: 26578768

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Coatomer subunit delta Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 270 amino acids
Theoretical weight: 30.02 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P43621 (Residues: 282-546; Coverage: 49%)
Gene names: RET2, YFR051C
ADP-ribosylation factor GTPase-activating protein GCS1 Chains: D, E
Molecule details ›
Chains: D, E
Length: 8 amino acids
Theoretical weight: 1.07 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P35197 (Residues: 345-352; Coverage: 2%)
Gene names: GCS1, YDL226C

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 15 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 70.765Å b: 84.855Å c: 148.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.197 0.287
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Phospholipid flippases in membrane remodeling and transport carrier biogenesis.
Best et al. (2019)
4 more