› 5fd9

X-ray diffraction
1.6Å resolution

X-ray Crystal Structure of ESCRT-III Snf7 core domain (conformation B)

Released:
DOI: 10.2210/pdb5fd9/pdb
PDB entry 5fd9 coloured by chain, front view.
PDB entry 5fd9 coloured by chain, side view.
PDB entry 5fd9 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural basis for activation, assembly and membrane binding of ESCRT-III Snf7 filaments.
Tang S, Henne WM, Borbat PP, Buchkovich NJ, Freed JH, Mao Y, Fromme JC, Emr SD
Elife 4 (2015)
PMID: 26670543

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153982 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vacuolar-sorting protein SNF7 Chain: A
Molecule details ›
Chain: A
Length: 139 amino acids
Theoretical weight: 15.8 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P39929 (Residues: 12-150; Coverage: 58%)
Gene names: DID1, SNF7, VPS32, YLR025W
Sequence domains: Snf7

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P21
Unit cell:
a: 29.901Å b: 46.201Å c: 44.601Å
α: 90° β: 98.5° γ: 90°
R-values:
R R work R free
0.211 0.21 0.225
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Reverse-topology membrane scission by the ESCRT proteins.
Schöneberg et al. (2017)
18 more

5 mentions without citation

Dividing the Archaeal Way: The Ancient Cdv Cell-Division Machinery.
Caspi et al. (2018)
4 more