5f74

X-ray diffraction
2.35Å resolution

Function and Biology Details

Structure analysis Details

Assemblies composition:
Non-polymer only dimer (preferred)
Non-polymer only octamer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein beta/alpha Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 28.12 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9CQV8 (Residues: 1-246; Coverage: 100%)
Gene name: Ywhab
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
Carbohydrate-responsive element-binding protein Chain: B
Molecule details ›
Chain: B
Length: 196 amino acids
Theoretical weight: 22.66 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8VIP2 (Residues: 1-196; Coverage: 23%)
Gene names: Mlxipl, Wbscr14

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P4222
Unit cell:
a: 115.62Å b: 115.62Å c: 59.372Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.188 0.255
Expression system: Escherichia coli