5f64

X-ray diffraction
2.71Å resolution

Putative positive transcription regulator (sensor EvgS) from Shigella flexneri

Released:
Entry authors: Nocek B, Osipiuk J, Mulligan R, Gu M, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-binding transcriptional activator EvgA Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 207 amino acids
Theoretical weight: 22.99 KDa
Source organism: Shigella flexneri 2a str. 2457T
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ACZ7 (Residues: 1-204; Coverage: 100%)
Gene names: S2573, SF2436, evgA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 168.485Å b: 110.867Å c: 110.769Å
α: 90° β: 126.74° γ: 90°
R-values:
R R work R free
0.185 0.183 0.223
Expression system: Escherichia coli BL21(DE3)