PDBe 5f38

X-ray diffraction
1.9Å resolution

X-ray crystal structure of a thiolase from Escherichia coli at 1.8 A resolution

Released:

Function and Biology Details

Reaction catalysed:
2 acetyl-CoA = CoA + acetoacetyl-CoA
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Acetyl-CoA acetyltransferase Chain: B
Molecule details ›
Chain: B
Length: 393 amino acids
Theoretical weight: 40.32 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P76461 (Residues: 1-393; Coverage: 100%)
Gene names: JW2218, atoB, b2224
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1
Acetyl-CoA acetyltransferase Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 40.43 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P76461 (Residues: 1-394; Coverage: 100%)
Gene names: JW2218, atoB, b2224
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1
Acetyl-CoA acetyltransferase Chain: D
Molecule details ›
Chain: D
Length: 394 amino acids
Theoretical weight: 40.36 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P76461 (Residues: 1-392; Coverage: 100%)
Gene names: JW2218, atoB, b2224
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1
Acetyl-CoA acetyltransferase Chain: C
Molecule details ›
Chain: C
Length: 395 amino acids
Theoretical weight: 40.48 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P76461 (Residues: 1-393; Coverage: 100%)
Gene names: JW2218, atoB, b2224
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments


Cofactor: Ligand COZ 1 x COZ
2 bound ligands:

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 73.794Å b: 76.254Å c: 266.607Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.235
Expression system: Escherichia coli