5f33

X-ray diffraction
1.45Å resolution

Structure of quinolinate synthase in complex with phosphoglycolohydroxamate

Released:

Function and Biology Details

Reaction catalysed:
Glycerone phosphate + iminosuccinate = pyridine-2,3-dicarboxylate + 2 H(2)O + phosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Quinolinate synthase A Chain: A
Molecule details ›
Chain: A
Length: 305 amino acids
Theoretical weight: 34.64 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9X1X7 (Residues: 1-298; Coverage: 100%)
Gene names: TM_1644, nadA
Sequence domains: Quinolinate synthetase A protein
Structure domains: NadA-like

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P21
Unit cell:
a: 52.094Å b: 49.16Å c: 60.221Å
α: 90° β: 104.1° γ: 90°
R-values:
R R work R free
0.144 0.141 0.186
Expression system: Escherichia coli BL21(DE3)