PDBe 5eg0

X-ray diffraction
3.1Å resolution

HOXB13-MEIS1 heterodimer bound to DNA

Released:
Source organism: Homo sapiens
Entry authors: Morgunova E, Yin Y, Jolma A, Popov A, Taipale J

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Homeobox protein Meis2 Chain: A
Molecule details ›
Chain: A
Length: 57 amino acids
Theoretical weight: 6.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14770 (Residues: 284-338; Coverage: 12%)
Gene names: MEIS2, MRG1
Sequence domains: Homeobox KN domain
Structure domains: Homeodomain-like
Homeobox protein Hox-B13 Chain: B
Molecule details ›
Chain: B
Length: 61 amino acids
Theoretical weight: 7.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92826 (Residues: 217-277; Coverage: 22%)
Gene name: HOXB13
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
DNA (5'-D(P*GP*TP*TP*GP*AP*CP*AP*GP*TP*TP*TP*TP*AP*CP*GP*AP*GP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 18 nucleotides
Theoretical weight: 5.59 KDa
DNA (5'-D(*CP*CP*TP*CP*GP*TP*AP*AP*AP*AP*CP*TP*GP*TP*CP*AP*AP*C)-3') Chain: E
Molecule details ›
Chain: E
Length: 18 nucleotides
Theoretical weight: 5.44 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 41.726Å b: 56.332Å c: 115.152Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.288 0.286 0.322
Expression system: Escherichia coli BL21(DE3)