5e63

X-ray diffraction
2.6Å resolution

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
1 distinct polypeptide molecule
4 distinct DNA molecules
Macromolecules (5 distinct):
I-SmaMI LAGLIDADG meganuclease Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 34.09 KDa
Source organism: Sordaria macrospora k-hell
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F7WD42 (Residues: 114-415; Coverage: 70%)
Gene name: SMAC_12671
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
DNA right half site Bottom strand Chain: B
Molecule details ›
Chain: B
Length: 14 nucleotides
Theoretical weight: 4.25 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA left half site bottom strand Chain: C
Molecule details ›
Chain: C
Length: 11 nucleotides
Theoretical weight: 3.4 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA left site top strand Chain: D
Molecule details ›
Chain: D
Length: 15 nucleotides
Theoretical weight: 4.53 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*CP*AP*GP*GP*TP*GP*TP*AP*CP*G)-3') Chain: E
Molecule details ›
Chain: E
Length: 10 nucleotides
Theoretical weight: 3.09 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 59.835Å b: 67.694Å c: 97.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.189 0.279
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided