5e5m

X-ray diffraction
2.18Å resolution

Crystal structure of mouse CTLA-4 in complex with nanobody

Released:
Source organisms:
Entry authors: Fedorov AA, Fedorov EV, Samanta D, Bonanno JB, Almo SC

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cytotoxic T-lymphocyte protein 4 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 117 amino acids
Theoretical weight: 12.93 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P09793 (Residues: 38-154; Coverage: 62%)
Gene names: Cd152, Ctla4
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
CTLA-4 nanobody Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 129 amino acids
Theoretical weight: 13.69 KDa
Source organism: Camelidae
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P212121
Unit cell:
a: 78.22Å b: 111.572Å c: 112.881Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.247
Expression system: Escherichia coli