X-ray diffraction
2.75Å resolution

Crystal structure of human 14-3-3 gamma in complex with CFTR R-domain peptide pS768-pS795


Function and Biology Details

Reaction catalysed:
ATP + H(2)O + closed Cl(-) channel = ADP + phosphate + open Cl(-) channel
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein gamma Chains: A, B, E, F, I, J
Molecule details ›
Chains: A, B, E, F, I, J
Length: 241 amino acids
Theoretical weight: 27.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P61981 (Residues: 1-238; Coverage: 96%)
Gene name: YWHAG
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
Cystic fibrosis transmembrane conductance regulator Chains: C, G, K
Molecule details ›
Chains: C, G, K
Length: 40 amino acids
Theoretical weight: 4.67 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: P13569 (Residues: 762-801; Coverage: 3%)
Gene names: ABCC7, CFTR

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P41212
Unit cell:
a: 122.45Å b: 122.45Å c: 313.159Å
α: 90° β: 90° γ: 90°
R R work R free
0.204 0.202 0.244
Expression systems:
  • Escherichia coli
  • Not provided