PDBe 5czo

X-ray diffraction
2.89Å resolution

Structure of S. cerevisiae Hrr25:Mam1 complex, form 2

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Casein kinase I homolog HRR25 Chains: A, B
Molecule details ›
Chains: A, B
Length: 395 amino acids
Theoretical weight: 46.25 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P29295 (Residues: 1-394; Coverage: 80%)
Gene names: HRR25, YPL204W
Sequence domains: Protein kinase domain
Monopolin complex subunit MAM1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 105 amino acids
Theoretical weight: 12.88 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P40065 (Residues: 87-191; Coverage: 35%)
Gene names: MAM1, YER106W

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: P21
Unit cell:
a: 52.701Å b: 83.841Å c: 132.508Å
α: 90° β: 92.57° γ: 90°
R-values:
R R work R free
0.22 0.218 0.253
Expression system: Escherichia coli