PDBe 5cyz

X-ray diffraction
1.84Å resolution

Structure of S. cerevisiae Hrr25:Mam1 complex, form 1

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Casein kinase I homolog HRR25 Chain: A
Molecule details ›
Chain: A
Length: 395 amino acids
Theoretical weight: 46.44 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P29295 (Residues: 1-394; Coverage: 80%)
Gene names: HRR25, YPL204W
Sequence domains: Protein kinase domain
Monopolin complex subunit MAM1 Chain: C
Molecule details ›
Chain: C
Length: 105 amino acids
Theoretical weight: 12.9 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P40065 (Residues: 87-191; Coverage: 35%)
Gene names: MAM1, YER106W

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 54.19Å b: 83.986Å c: 67.424Å
α: 90° β: 108.53° γ: 90°
R-values:
R R work R free
0.165 0.163 0.192
Expression system: Escherichia coli